Hoshyar Reyhane, Mahboob Zahra, Zarban Asghar
Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, P.O. Box: 9717853577, Birjand, Iran.
Cytotechnology. 2016 Aug;68(4):1207-13. doi: 10.1007/s10616-015-9880-y. Epub 2015 Apr 29.
In this study we evaluated the biological activity of alcoholic and aqueous extracts from the fruit of Berberis vulgaris. The total antioxidant capacity of Berberis was characterized by FRAP, DPPH, Folin-Ciocalteu while the anthocyanins content was measured by pH differential method. Cell viability and apoptotic property were determined by MTT and DNA fragmentation assays, respectively. Alcoholic extract of Berberis was richer in antioxidants and anthocyanins compared to aqueous extract. Although both extracts significantly inhibited proliferation of breast cancer cells (MCF-7); these changes were not observed in normal human breast epithelial cells (MCF10-A). The alcoholic extract was more effective in inducing apoptosis as detected by DNA fragmentation in treated cancer cells. Our results suggest that Berberis has potent antioxidant properties and cytotoxic effects that can induce apoptosis. Therefore, Berberis can potentially be exploited for the development of therapeutics to fight against human breast cancer.
在本研究中，我们评估了普通小檗果实的乙醇提取物和水提取物的生物活性。采用铁离子还原抗氧化能力（FRAP）、1,1-二苯基-2-三硝基苯肼（DPPH）、福林-酚法测定小檗的总抗氧化能力，同时采用pH差值法测定花色苷含量。分别通过MTT法和DNA片段化分析测定细胞活力和凋亡特性。与水提取物相比，小檗乙醇提取物中的抗氧化剂和花色苷含量更高。尽管两种提取物均能显著抑制乳腺癌细胞（MCF-7）的增殖，但在正常人乳腺上皮细胞（MCF10-A）中未观察到这些变化。通过处理后癌细胞的DNA片段化检测发现，乙醇提取物在诱导细胞凋亡方面更有效。我们的结果表明，小檗具有强大的抗氧化特性和细胞毒性作用，能够诱导细胞凋亡。因此，小檗有可能被用于开发对抗人类乳腺癌的治疗药物。
