Mansour Rym, Haouas Najoua, Ben Kahla-Nakbi Amel, Hammami Saoussen, Mighri Zine, Mhenni Farouk, Babba Hamouda
Unité of Recherche 12-04, Chimie Appliquée and Environnement, Faculté of Sciences of Monastir, Monastir 5000, Tunisie.
Iran J Pharm Res. 2013 Summer;12(3):349-55.
Vitis vinifera L.is a traditional Asian herb widely used for different health problems. In the present research, the ethanolic and the aqueous extracts of Vitis vinifera L. leaves collected from shrub, grown in Tunisia, were prepared and evaluated for the antileishmanial activity against Leishmani ainfantum promastigotes. The inhibitory concentration 50 (IC50) was determined and the results showed that the etahnolic extract is more active than the aqueous one (IC50= 0.108 mg/mL). Microscopic observations showed that the ethanolic extract promoted the destruction of cytoplasmic and nuclear membranes of Leishmani ainfantum promastigotes and altered the overall shape of the cell. In order to explain the difference of antileishmanial activity between ethanolic and aqueous extracts, anthocyanins amount was determined by spectrophotometry. It was found that the ethanolic extract is richer in anthocyanins than the aqueous one which can explain the higher antileishmanial activity of the ethanolic extract.
葡萄（Vitis vinifera L.）是一种传统的亚洲草药，广泛用于治疗各种健康问题。在本研究中，制备了从突尼斯种植的灌木上采集的葡萄叶的乙醇提取物和水提取物，并评估了它们对婴儿利什曼原虫前鞭毛体的抗利什曼活性。测定了半数抑制浓度（IC50），结果表明乙醇提取物比水提取物更具活性（IC50 = 0.108 mg/mL）。显微镜观察表明，乙醇提取物促进了婴儿利什曼原虫前鞭毛体细胞质膜和核膜的破坏，并改变了细胞的整体形状。为了解释乙醇提取物和水提取物之间抗利什曼活性的差异，通过分光光度法测定了花青素含量。发现乙醇提取物中的花青素比水提取物中的更丰富，这可以解释乙醇提取物具有更高抗利什曼活性的原因。
