Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Daneshgah street 51666-14766, Tabriz, Iran.
Bosn J Basic Med Sci. 2013 Aug;13(3):186-91. doi: 10.17305/bjbms.2013.2360.
Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan's test at a significance level of P≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma.
新型治疗方法的开发对于口腔鳞状细胞癌（OSCC）的治疗至关重要。最近的科学研究集中在草药作为潜在的抗癌药物候选物上。本研究首次探讨了葡萄籽提取物（GSE）在口腔鳞状细胞癌（KB 细胞）中诱导细胞毒性作用和细胞死亡的机制。MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐）和台盼蓝测定法用于 KB 细胞以及人脐静脉内皮细胞（HUVEC）以分析 GSE 的细胞毒性活性。此外，通过 TUNEL、DNA 片段化和细胞死亡分析来确定提取物的凋亡诱导作用。通过方差分析（ANOVA）确定统计学意义，然后在 P≤0.05 的显着性水平下进行 Duncan 检验。结果表明 GSE 具有潜在的凋亡作用，通过在不诱导非癌细胞系 HUVEC 损伤的情况下，以剂量和时间依赖性方式显著抑制细胞生长和活力得到证实。本研究结果表明，这种植物可能含有治疗口腔鳞状细胞癌的潜在生物活性化合物。
