Cvetkovic Tatjana, Stankovic Jablan, Najman Stevo, Pavlovic Dusica, Stokanovic Dragana, Vlajkovic Slobodan, Dakovic-Bjelakovic Marija, Cukuranovic Jovana, Zivkovic Vladimir, Stefanovic Vladisav
Department of Biochemistry, School of Medicine, University of Nis, Nis, Serbia ; Clinic of Nephrology, Clinical Centre Nis, Nis, Serbia.
Clinic of Urology, Clinical Centre Nis, Nis, Serbia.
Int J Fertil Steril. 2015 Apr-Jun;9(1):121-8. doi: 10.22074/ijfs.2015.4216. Epub 2015 Apr 21.
The aim of this study was to determine oxidative stress (OS) parameters after testicular torsion/detorsion in adult rats.
In this experimental study, male adult Wistar rats were divided into four groups, each consisting of seven animals: group I-one hour right testicular torsion with subsequent orchiectomy, group II-one hour right testicular torsion followed by detorsion, group III-unilateral right-sided orchiectomy without previous torsion and group IV-control. After 30 days, bilateral orchiectomies were performed in rats with both testes and unilateral orchiectomies in rats with single testicles. Parameters of OS were determined in testicular tissue and in plasma.
Plasma concentrations of advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS) were higher (p<0.05 and p<0.01, respectively), whilst the plasma concentration of the total sulfhydryl (T-SH)-groups was lower (p<0.05) in group I compared to the control group. Group II had higher plasma concentrations of AOPP compared to group IV (p<0.05), as well as significantly increased TBARS and decreased T-SH-group levels compared to groups III (p<0.05 and p<0.01, respectively) and IV (p<0.01, for both parameters). There were significant differences in OS markers between the ipsilateral and contralateral testis, as well as significant correlations among levels of both plasma and tissue markers of OS.
The increase in TBARS levels seen throughout the experimental period indicated that OS development was caused by ischemia/reperfusion in the testicular tissue. The oxidant-antioxidant system of the testicular tissue was altered during torsion as well as detorsion.
本研究旨在确定成年大鼠睾丸扭转/复位后的氧化应激（OS）参数。
在本实验研究中，将成年雄性Wistar大鼠分为四组，每组七只动物：第一组——右侧睾丸扭转1小时后行睾丸切除术；第二组——右侧睾丸扭转1小时后再复位；第三组——未预先扭转而行单侧右侧睾丸切除术；第四组——对照组。30天后，对双侧睾丸的大鼠进行双侧睾丸切除术，对单睾丸大鼠进行单侧睾丸切除术。测定睾丸组织和血浆中的OS参数。
与对照组相比，第一组血浆中晚期氧化蛋白产物（AOPP）和硫代巴比妥酸反应性物质（TBARS）的浓度较高（分别为p<0.05和p<0.01），而总巯基（T-SH）基团的血浆浓度较低（p<0.05）。与第四组相比，第二组血浆中AOPP的浓度较高（p<0.05），与第三组（分别为p<0.05和p<0.01）和第四组（两个参数均为p<0.01）相比，TBARS显著升高，T-SH基团水平显著降低。同侧和对侧睾丸之间的OS标志物存在显著差异，血浆和组织OS标志物水平之间也存在显著相关性。
在整个实验期间观察到的TBARS水平升高表明，OS的发生是由睾丸组织的缺血/再灌注引起的。睾丸组织的氧化-抗氧化系统在扭转和复位过程中均发生了改变。
