与苏丹病毒连续传代相关的糖蛋白编辑位点的基因变化
Genetic Changes at the Glycoprotein Editing Site Associated With Serial Passage of Sudan Virus.
作者信息
Alfson Kendra J, Avena Laura E, Beadles Michael W, Menzie Heather, Patterson Jean L, Carrion Ricardo, Griffiths Anthony
机构信息
Department of Virology and Immunology, Texas Biomedical Research Institute Department of Microbiology and Immunology, University of Texas Health Science Center, San Antonio.
Department of Virology and Immunology, Texas Biomedical Research Institute.
出版信息
J Infect Dis. 2015 Oct 1;212 Suppl 2(Suppl 2):S295-304. doi: 10.1093/infdis/jiv216. Epub 2015 Apr 28.
Abstract
Sudan virus (SUDV), like the closely related Ebola virus (EBOV), is a filovirus that causes severe hemorrhagic disease. They both contain an RNA editing site in the glycoprotein gene that controls expression of soluble and full-length protein. We tested the consequences of cell culture passage on the genome sequence at the SUDV editing site locus and determined whether this affected virulence. Passage resulted in expansion of the SUDV editing site, similar to that observed with EBOV. We compared viruses possessing either the wild-type or expanded editing site, using a nonhuman primate model of disease. Despite differences in virus serum titer at one time point, there were no significant differences in time to death or any other measured parameter. These data imply that changes at this locus were not important for SUDV lethality.
摘要
苏丹病毒（SUDV）与密切相关的埃博拉病毒（EBOV）一样，是一种能引起严重出血性疾病的丝状病毒。它们在糖蛋白基因中都含有一个RNA编辑位点，该位点控制可溶性蛋白和全长蛋白的表达。我们测试了细胞培养传代对SUDV编辑位点基因座基因组序列的影响，并确定这是否会影响病毒毒力。传代导致SUDV编辑位点扩增，这与在EBOV中观察到的情况类似。我们使用疾病的非人灵长类动物模型比较了具有野生型或扩增编辑位点的病毒。尽管在一个时间点病毒血清滴度存在差异，但在死亡时间或任何其他测量参数上没有显著差异。这些数据表明，该位点的变化对SUDV致死性并不重要。
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