†Laboratory of Food Chemistry, Wageningen University , P.O. Box 17, 6700 AA Wageningen, The Netherlands.
‡Faculty of Veterinary Medicine, Institute for Risk Assessment Sciences, Subdivision of Veterinary Pharmacology, Pharmacotherapy, and Toxicology, Utrecht University, 3584 CM Utrecht, The Netherlands.
J Agric Food Chem. 2015 May 20;63(19):4805-14. doi: 10.1021/acs.jafc.5b01127. Epub 2015 May 8.
Colostrum oligosaccharides are known to exhibit prebiotic and immunomodulatory properties. Oligosaccharide composition is species-specific, and equine colostrum has been reported to contain unique oligosaccharides. Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation. Sixteen EMOS were characterized and quantified, of which half were neutral and half were acidic. EMOS showed about 63% structural overlap with human milk oligosaccharides, known for their bioactivity. Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified). In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively). Differences in presence and in abundance of specific EMOS were evident not only between the four breeds but also within the breed.
初乳寡糖已知具有益生元和免疫调节特性。寡糖组成具有物种特异性，马初乳已被报道含有独特的寡糖。因此，通过 CE-LIF、CE-MS(n)、HILIC-MS(n) 和外切糖苷酶降解分析了来自不同马品种的初乳的马源寡糖（EMOS）。鉴定和定量了 16 种 EMOS，其中一半为中性，一半为酸性。EMOS 与以生物活性而闻名的人乳寡糖具有约 63%的结构重叠。有 7 种 EMOS 以前在马源寡糖文献中没有报道过：中性 Gal(β1-4)HexNAc、Gal(β1-4)Hex-Hex、β4'-半乳糖基乳糖和乳糖-N-六糖，以及酸性 6'-唾液酸-Hex-Ac-HexNAc、唾液酸化乳糖-N-四糖-a 和二唾液酸化乳糖-N-四糖（未进一步指定异构体）。在所有初乳样本中，平均寡糖浓度范围为 2.12 至 4.63 g/L；β 6'和 3'-半乳糖基乳糖、3'-唾液酸乳糖和二唾液酸乳糖是所有寡糖中最丰富的（分别为 27-59%、16-37%、1-8%和 1-6%）。不仅在四个品种之间，而且在品种内，特定 EMOS 的存在和丰度的差异都很明显。
