Waltham Petcare Science Institute, Waltham-on the-Wolds, Leicestershire, United Kingdom.
Department of Chemistry, University of California-Davis, Davis, California, United States of America.
PLoS One. 2020 Dec 3;15(12):e0243323. doi: 10.1371/journal.pone.0243323. eCollection 2020.
Oligosaccharides are important components of milk, serving as substrates for the intestinal microbiota, acting as antimicrobials that prevent pathogen colonization, and supporting the developing gastrointestinal immune system of neonates. Nutrient composition of canine and feline milk samples has been described previously, but little is known about the oligosaccharide content. Therefore, the objective of this study was to characterize canine and feline milk samples using a high-throughput glycomics approach. 23 dogs (9 Labrador retriever and 14 Labrador retriever x golden retriever crossbreed) and 6 domestic shorthair cats were recruited to the study. Milk samples were collected by manual expression at time points after parturition. Samples were collected across 2 phases per species, differentiated by maternal diet. Following extraction, oligosaccharide content was determined by liquid chromatography-mass spectrometry (LC-MS). In canine milk samples, 3 structures accounted for over 90% of all oligosaccharides detected across two diet groups. These were 3'-sialyllactose, 6'-sialyllactose, and 2'-fucosyllactose. In feline samples, a more diverse range of oligosaccharides was detected, with up to 16 structures present at relative abundance >1% of the total. Difucosyllactose-N-hexaose b, 3'-sialyllactose and lacto-N-neohexaose were all detected at abundances >10% in feline milk samples. Statistically significant differences (p<0.05) in oligosaccharide abundances were observed between collection time points and between diet groups within species. These data explore the oligosaccharide content of canine and feline maternal milk, representing an opportunity to generate a fundamental understanding of the nutritional needs of new-born puppies and kittens.
寡糖是牛奶的重要组成部分，它们是肠道微生物群的底物，具有抗微生物作用，可以防止病原体定植，并支持新生儿胃肠道免疫系统的发育。犬和猫的奶样的营养成分以前已经有过描述，但对于寡糖的含量知之甚少。因此，本研究的目的是使用高通量糖组学方法对犬和猫的奶样进行特征描述。招募了 23 只狗（9 只拉布拉多猎犬和 14 只拉布拉多猎犬 x 金毛猎犬杂交）和 6 只家短毛猫参与研究。在产后时间点通过手动表达收集奶样。每只动物采集 2 个样本，每个样本分两个阶段采集，由母体饮食区分。样本提取后，通过液相色谱-质谱法（LC-MS）测定寡糖含量。在犬奶样中，3 种结构占两个饮食组检测到的所有寡糖的 90%以上。这些结构是 3'-唾液乳糖、6'-唾液乳糖和 2'-岩藻糖乳糖。在猫的样本中，检测到更多种类的寡糖，多达 16 种结构的相对丰度超过总寡糖的 1%。双岩藻糖乳糖-N-六糖 b、3'-唾液乳糖和乳-N-新六糖都在猫奶样中的丰度超过 10%。在物种内的采集时间点和饮食组之间观察到寡糖丰度的统计学显著差异（p<0.05）。这些数据探索了犬和猫的母乳中的寡糖含量，为了解新出生的小狗和小猫的营养需求提供了机会。
