Owen C S
Department of Biochemistry and Molecular Biology, Jefferson Medical College, Philadelphia, PA 19107.
J Immunogenet. 1989 Apr;16(2):117-23. doi: 10.1111/j.1744-313x.1989.tb00453.x.
Magnetic filtration of labelled cells as a way of separating leucocyte subpopulations was tested with a very simple and easy filtration device, using colloidal magnetite as the labelling reagent. In order to quantitate cell enrichment, a double label (both fluorescent and magnetic) was used, under conditions which labelled less than 10% of the cells in the initial sample. Up to 20 million cells were simply passed through a small magnetic filter with a hand-held syringe. Depletion of labelled cells in the suspension that passed through was threefold, and enrichment of labelled cells in the wash of the filter after its removal from the magnet was approximately fivefold. Factors which limited the quality of separation are discussed. Other, more preliminary, experiments found enrichments of 15- to 30-fold with the same colloidal magnetite and hand-held apparatus when the cell labelling system was more selective.
使用一种非常简单且易于操作的过滤装置，以胶体磁铁矿作为标记试剂，对通过磁性过滤标记细胞来分离白细胞亚群的方法进行了测试。为了对细胞富集进行定量，在标记初始样品中不到10%细胞的条件下，使用了双重标记（荧光和磁性）。多达2000万个细胞通过手持注射器简单地通过一个小型磁性过滤器。通过的悬浮液中标记细胞的损耗为三倍，从磁铁上取下过滤器后的洗液中标记细胞的富集约为五倍。讨论了限制分离质量的因素。其他更初步的实验发现，当细胞标记系统更具选择性时，使用相同的胶体磁铁矿和手持设备可实现15至30倍的富集。
