Instrumental Analysis Center, Dalian Polytechnic University, Dalian 116034, PR China; School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, PR China.
Instrumental Analysis Center, Dalian Polytechnic University, Dalian 116034, PR China.
Colloids Surf B Biointerfaces. 2015 Jun 1;130:299-304. doi: 10.1016/j.colsurfb.2015.04.033. Epub 2015 Apr 22.
We report a novel method to synthesize organic-inorganic nanoflowers for crude soybean peroxidase (SBP) purification. A hierarchical flower-like spherical structure with hundreds of nanopetals was self-assembled by using crude SBP as the organic component and Cu3(PO4)2·3H2O as the inorganic component. The structure of the hybrid nanoflowers was confirmed by Fourier-transform infrared spectroscopy, X-ray diffraction, and energy-dispersive X-ray spectroscopy, and the enzymatic activity of SBP embedded in the hybrid nanoflowers was evaluated using guaiacol as substrate. Compared with free crude SBP in solution, SBP embedded in hybrid nanoflowers exhibited enhanced enzymatic activity (∼446%). The hybrid nanoflowers also exhibited excellent reusability and reproducibility during cycle analysis. These results demonstrate that synthesis of hybrid nanoflowers is an effective enzyme purification strategy.
我们报告了一种新颖的方法来合成有机-无机纳米花，用于粗大豆过氧化物酶（SBP）的纯化。一种具有数百个纳米花瓣的分层花状球形结构是通过使用粗 SBP 作为有机成分和 Cu3(PO4)2·3H2O 作为无机成分自组装而成的。通过傅里叶变换红外光谱、X 射线衍射和能谱分析证实了混合纳米花的结构，并使用愈创木酚作为底物评估了嵌入混合纳米花中的 SBP 的酶活性。与溶液中的游离粗 SBP 相比，嵌入混合纳米花中的 SBP 表现出增强的酶活性（约 446%）。在循环分析过程中，混合纳米花还表现出优异的可重复使用性和重现性。这些结果表明，混合纳米花的合成是一种有效的酶纯化策略。
