McDonough Ashley, Monterrubio Angela, Ariza Jeanelle, Martínez-Cerdeño Verónica
Department of Pathology and Laboratory Medicine, University of California, Davis; Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children (Northern California).
Department of Pathology and Laboratory Medicine, University of California, Davis; Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children (Northern California);
J Vis Exp. 2015 Apr 24(98):52318. doi: 10.3791/52318.
Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The calibrated forceps model of compression injury is a convenient, low cost, and very reproducible animal model for SCI. We used a pair of modified forceps in accordance with the method published by Plemel et al. (2008) to laterally compress the spinal cord to a distance of 0.35 mm. In this video, we will demonstrate a dorsal laminectomy to expose the spinal cord, followed by compression of the spinal cord with the modified forceps. In the video, we will also address issues related to the care of paraplegic laboratory animals. This injury model produces mice that exhibit impairment in sensation, as well as impaired hindlimb locomotor function. Furthermore, this method of injury produces consistent aberrations in the pathology of the SCI, as determined by immunohistochemical methods. After watching this video, viewers should be able to determine the necessary supplies and methods for producing SCI of various severities in the mouse for studies on SCI and/or treatments designed to mitigate impairment after injury.
小鼠脊髓压迫损伤是用于研究脊髓损伤（SCI）和脊髓再生治疗的有价值的动物模型。校准镊子压迫损伤模型是一种用于SCI研究的便捷、低成本且高度可重复的动物模型。我们按照Plemel等人（2008年）发表的方法使用一对改良镊子将脊髓侧向压迫至0.35毫米的距离。在本视频中，我们将展示背部椎板切除术以暴露脊髓，随后用改良镊子压迫脊髓。在视频中，我们还将讨论与截瘫实验动物护理相关的问题。这种损伤模型产生的小鼠表现出感觉障碍以及后肢运动功能受损。此外，通过免疫组织化学方法确定，这种损伤方法在SCI病理学中产生一致的异常。观看本视频后，观众应能够确定在小鼠中产生各种严重程度的SCI以用于SCI研究和/或旨在减轻损伤后功能障碍的治疗研究所需的用品和方法。
