Nano-food Research Group, Instrumental and Food Analysis Laboratory, Industrial Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.
Research Wing, Veer Kunwar Singh Memorial Trust, Chapra, Bihar, India.
Environ Sci Pollut Res Int. 2016 Mar;23(5):4149-63. doi: 10.1007/s11356-015-4570-z. Epub 2015 May 6.
In recent years, silver nanoparticles (AgNPs) have attracted considerable interest in the field of food, agriculture and pharmaceuticals mainly due to its antibacterial activity. AgNPs have also been reported to possess toxic behavior. The toxicological behavior of nanomaterials largely depends on its size and shape which ultimately depend on synthetic protocol. A systematic and detailed analysis for size variation of AgNP by thermal co-reduction approach and its efficacy toward microbial and cellular toxicological behavior is presented here. With the focus to explore the size-dependent toxicological variation, two different-sized NPs have been synthesized, i.e., 60 nm (Ag60) and 85 nm (Ag85). A detailed microbial toxicological evaluation has been performed by analyzing minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), diameter of inhibition zone (DIZ), growth kinetics (GrK), and death kinetics (DeK). Comparative cytotoxicological behavior was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. It has been concluded by this study that the size of AgNPs can be varied, by varying the concentration of reactants and temperature called as "thermal co-reduction" approach, which is one of the suitable approaches to meet the same. Also, the smaller AgNP has shown more microbial and cellular toxicity.
近年来，由于银纳米粒子（AgNPs）具有抗菌活性，因此在食品、农业和制药领域引起了相当大的关注。据报道，AgNPs 还具有毒性行为。纳米材料的毒理学行为在很大程度上取决于其尺寸和形状，而这最终取决于合成方案。本文提出了一种通过热共还原方法对 AgNP 尺寸变化进行系统和详细分析及其对微生物和细胞毒理学行为的功效的方法。为了探索尺寸依赖性的毒理学变化，我们合成了两种不同尺寸的 NPs，即 60nm（Ag60）和 85nm（Ag85）。通过分析最小抑菌浓度（MIC）、最小杀菌浓度（MBC）、抑菌圈直径（DIZ）、生长动力学（GrK）和死亡动力学（DeK），对其进行了详细的微生物毒理学评价。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物（MTT）测定法分析了比较细胞毒性行为。通过这项研究得出结论，AgNPs 的尺寸可以通过改变反应物的浓度和温度（称为“热共还原”方法）来改变，这是满足这一需求的合适方法之一。此外，较小的 AgNP 显示出更强的微生物和细胞毒性。
