Tung Bui Thanh, Rodriguez-Bies Elisabet, Thanh Hai Nguyen, Le-Thi-Thu Huong, Navas Plácido, Sanchez Virginia Motilva, López-Lluch Guillermo
School of Medicine and Pharmacy, Vietnam National University, Hanoi, Floor 5 Building Y1, 144 Xuan Thuy, Cau Giay, Hanoi, Vietnam.
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC, CIBERER, Instituto de Salud Carlos III, Carretera de Utrera Km. 1, 41013, Seville, Spain.
Aging Clin Exp Res. 2015 Dec;27(6):775-83. doi: 10.1007/s40520-015-0366-8. Epub 2015 May 8.
Oxidative stress has been considered one of the causes of aging. For this reason, treatments based on antioxidants or those capable of increasing endogenous antioxidant activity have been taken into consideration to delay aging or age-related disease progression.
In this paper, we determine if resveratrol and exercise have similar effect on the antioxidant capacity of different organs in old mice.
Resveratrol (6 months) and/or exercise (1.5 months) was administered to old mice. Markers of oxidative stress (lipid peroxidation and glutathione) and activities and levels of antioxidant enzymes (SOD, catalase, glutathione peroxidase, glutathione reductase and transferase and thioredoxin reductases, NADH cytochrome B5-reductase and NAD(P)H-quinone acceptor oxidoreductase) were determined by spectrophotometry and Western blotting in different organs: liver, kidney, skeletal muscle, heart and brain.
Both interventions improved antioxidant activity in the major organs of the mice. This induction was accompanied by a decrease in the level of lipid peroxidation in the liver, heart and muscle of mice. Both resveratrol and exercise modulated several antioxidant activities and protein levels. However, the effect of resveratrol, exercise or their combination was organ dependent, indicating that different organs respond in different ways to the same stimulus.
Our data suggest that physical activity and resveratrol may be of great importance for the prevention of age-related diseases, but that their organ-dependent effect must be taken into consideration to design a better intervention.
氧化应激被认为是衰老的原因之一。因此，基于抗氧化剂或能够增加内源性抗氧化活性的治疗方法已被考虑用于延缓衰老或与年龄相关的疾病进展。
在本文中，我们确定白藜芦醇和运动对老年小鼠不同器官的抗氧化能力是否有相似的影响。
对老年小鼠给予白藜芦醇（6个月）和/或运动（1.5个月）。通过分光光度法和蛋白质印迹法测定不同器官（肝脏、肾脏、骨骼肌、心脏和大脑）中的氧化应激标志物（脂质过氧化和谷胱甘肽）以及抗氧化酶的活性和水平（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和转移酶以及硫氧还蛋白还原酶、NADH细胞色素B5还原酶和NAD(P)H-醌受体氧化还原酶）。
两种干预措施均改善了小鼠主要器官的抗氧化活性。这种诱导伴随着小鼠肝脏、心脏和肌肉中脂质过氧化水平的降低。白藜芦醇和运动均调节了多种抗氧化活性和蛋白质水平。然而，白藜芦醇、运动或它们的组合的效果因器官而异，表明不同器官对相同刺激的反应方式不同。
我们的数据表明，体育活动和白藜芦醇对于预防与年龄相关的疾病可能非常重要，但在设计更好的干预措施时必须考虑它们的器官依赖性效应。
