Zhao Jun-Long, Wang Yun-Li, Yao Dan-Qing, Zhu Wen-Ying, Chen Long, He Huan-Le, Pan Jun-Song, Cai Run
School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.
Shanghai Seed Management Station, 628 Wuzhong Road, Minhang District, Shanghai, 201103, China.
Mol Genet Genomics. 2015 Oct;290(5):2007-18. doi: 10.1007/s00438-015-1057-z. Epub 2015 May 8.
Trichomes on plants, similar to fine hairs on animal and human bodies, play important roles in plant survival and development. They also represent a useful model for the study of cell differentiation. Although the regulatory gene network of unicellular trichome development in Arabidopsis thaliana has been well studied, the genes that regulate multicellular trichome development remain unclear. We confirmed that Cucumis sativus (cucumber) trichomes are multicellular and unbranched, but identified a spontaneous mutant, trichome-less (tril), which presented a completely glabrous phenotype. We compared the transcriptome profilings of the tril mutant and wild type using the Illumina HiSeq 2000 sequencing technology. A total of 991 genes exhibited differential expression: 518 were up-regulated and 473 were down-regulated. We further identified 62 differentially expressed genes that encoded crucial transcription factors and were subdivided into seven categories: homeodomain, MADS, MYB, and WRKY domains, ethylene-responsive, zinc finger, and other transcription factor genes. We further analyzed the tissue-expression profiles of two candidate genes, GLABRA2-like and ATHB51-like, using qRT-PCR and found that these two genes were specifically expressed in the epidermis and trichomes, respectively. These results and the tril mutant provide useful tools to study the molecular networks associated with multicellular trichome development.
植物上的毛状体类似于动物和人体上的细毛，在植物的生存和发育中发挥着重要作用。它们也是研究细胞分化的有用模型。尽管拟南芥单细胞毛状体发育的调控基因网络已得到充分研究，但调控多细胞毛状体发育的基因仍不清楚。我们证实黄瓜的毛状体是多细胞且无分支的，但鉴定出一个自发突变体，无毛（tril），其表现出完全无毛的表型。我们使用Illumina HiSeq 2000测序技术比较了tril突变体和野生型的转录组图谱。共有991个基因表现出差异表达：518个上调，473个下调。我们进一步鉴定出62个差异表达基因，这些基因编码关键转录因子，并细分为七类：同源结构域、MADS、MYB和WRKY结构域、乙烯响应、锌指和其他转录因子基因。我们使用qRT-PCR进一步分析了两个候选基因GLABRA2-like和ATHB51-like的组织表达谱，发现这两个基因分别在表皮和毛状体中特异性表达。这些结果以及tril突变体为研究与多细胞毛状体发育相关的分子网络提供了有用的工具。
