Kinsey-Jones James S, Alamshah Amin, McGavigan Anne K, Spreckley Eleanor, Banks Katherine, Cereceda Monteoliva Nicholas, Norton Mariana, Bewick Gavin A, Murphy Kevin G
Section of Investigative Medicine, Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK.
Division of Diabetes and Nutritional Sciences, King's College London, Guy's Campus, London, UK.
Obesity (Silver Spring). 2015 Jun;23(6):1194-200. doi: 10.1002/oby.21083. Epub 2015 May 9.
The G-protein coupled receptor family C group 6 member A (GPRC6A) is activated by proteinogenic amino acids and may sense amino acids in the gastrointestinal tract and the brain. The study investigated whether GPRC6A was necessary for the effects of low- and high-protein diets on body weight and food intake in mice.
The role of GPRC6A in mediating the effects of a low-protein diet on body weight was investigated in GPRC6a knockout (GPRC6a-KO) and wild-type (WT) mice fed a control diet (18% protein) or a low-protein diet (6% protein) for 9 days. The role of GPRC6A in mediating the effects of a high-protein diet on body weight was investigated in GPRC6a-KO and WT mice fed a control diet (18% protein) or a high-protein diet (50% protein) for 5 weeks.
A high-protein diet reduced body weight gain and food intake compared with a control diet in both WT and GPRC6a-KO mice. A low-protein diet decreased body weight gain in GPRC6a-KO mice.
GPRC6A was not necessary for the effects of a low- or high-protein diet on body weight and likely does not play a role in protein-induced satiety.
G蛋白偶联受体C家族第6组成员A（GPRC6A）由蛋白质氨基酸激活，可能感知胃肠道和大脑中的氨基酸。本研究调查了GPRC6A对于低蛋白和高蛋白饮食对小鼠体重和食物摄入量影响是否必要。
在喂食对照饮食（18%蛋白质）或低蛋白饮食（6%蛋白质）9天的GPRC6a基因敲除（GPRC6a-KO）和野生型（WT）小鼠中，研究GPRC6A在介导低蛋白饮食对体重影响中的作用。在喂食对照饮食（18%蛋白质）或高蛋白饮食（50%蛋白质）5周的GPRC6a-KO和WT小鼠中，研究GPRC6A在介导高蛋白饮食对体重影响中的作用。
与对照饮食相比，高蛋白饮食在WT和GPRC6a-KO小鼠中均降低了体重增加和食物摄入量。低蛋白饮食降低了GPRC6a-KO小鼠的体重增加。
GPRC6A对于低蛋白或高蛋白饮食对体重的影响并非必要，可能在蛋白质诱导的饱腹感中不起作用。
