Pan Fan, Yu Tian-Yuan, Wong Steven, Xian Si-Tong, Lu Meng-Qian, Wu Jian-Cong, Gao Yu-Feng, Li Xiao-Qin, Geng Nan, Yao Bin-Bin
Collage of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, 100029, China.
The 2nd Massage Department, Beijing Massage Hospital, Beijing, 100035, China.
Chin J Integr Med. 2017 Aug;23(8):617-624. doi: 10.1007/s11655-015-2142-1. Epub 2015 May 13.
To elucidate the mechanism of Chinese tuina in treating sciatic nerve crush injury, and to detect the levels of tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1), which is thought to play an important role in nerve regeneration.
Thirty-two adult male Sprague-Dawley rats were subjected to sciatic nerve crush injury and 16 rats (sham-operated group) went through a sham operation. Control group was given no treatment while tuina group received tuina therapy since day 7 post-surgery. Tuina treatment was performed once a day and lasted for 20 days. The sciatic functional index was examined every 5 days during the treatment session. The rats' gastrocnemius muscles were evaluated for changes in mass and immunohistochemistry techniques were performed to detect the levels of tPA and PAI-1.
Tuina therapy improved the motor function of sciatic nerve injured rats (P<0.05), however, it did not increase muscle volume (P<0.05). Tuina downregulated the levels of tPA and PAI-1 (P<0.05).
The present study implies that tuina treatment could accelerate rehabilitation of peripheral nerve injury.
阐明中医推拿治疗坐骨神经挤压伤的机制，并检测组织型纤溶酶原激活物（tPA）和纤溶酶原激活物抑制剂-1（PAI-1）的水平，这两种物质被认为在神经再生中起重要作用。
32只成年雄性Sprague-Dawley大鼠接受坐骨神经挤压伤，16只大鼠（假手术组）进行假手术。对照组不进行治疗，而推拿组自术后第7天开始接受推拿治疗。推拿治疗每天进行1次，持续20天。治疗期间每5天检查坐骨神经功能指数。评估大鼠腓肠肌的质量变化，并采用免疫组织化学技术检测tPA和PAI-1的水平。
推拿治疗改善了坐骨神经损伤大鼠的运动功能（P<0.05），然而，它并未增加肌肉体积（P<0.05）。推拿下调了tPA和PAI-1的水平（P<0.05）。
本研究表明推拿治疗可加速周围神经损伤的康复。
