Özdemir Fatma, Arslan Seza
Abant Izzet Baysal Univ, Faculty of Arts and Sciences, Dept. of Biology, 14280, Gölköy/Bolu, Turkey.
J Food Sci. 2015 Jun;80(6):M1306-13. doi: 10.1111/1750-3841.12911. Epub 2015 May 12.
A total of 300 food samples including 180 milk and 120 meat products have been examined for the presence of Yersinia spp. using the ISO 10273 and the cold enrichment method. The overall prevalence of Yersinia spp. was 84 (28%). Yersinia enterocolitica was isolated from 18 (6%) of the 300 samples. The other Yersinia species were detected in the samples Yersinia rohdei 15 (5%), Yersinia intermedia 14 (4.7%), Yersinia pseudotuberculosis 12 (4%), Yersinia ruckeri 12 (4%), Yersinia mollaretii 5 (1.7%), Yersinia bercovieri 4 (1.3%), and atypical Yersinia spp. 4 (1.3%). The conventionally identified Y. enterocolitica strains were also confirmed by the 16S rRNA gene sequencing. All Y. enterocolitica strains biotyped as 1A had negative results in the phenotypic virulence tests. The 84 Yersinia strains were also examined genotypically for the presence of virulence genes. None of the Y. enterocolitica and other Yersinia strains contained the ail, ystA, yadA, and virF except only 1 Y. intermedia and 2 Y. enterocolitica strains that were found to be positive for ystB. Antimicrobial resistance of 84 Yersinia to 16 antimicrobial agents was determined by the disk diffusion method. All strains were sensitive to tobramycine and imipenem while resistant to clindamycin. Although 84.5% of the strains were resistant to at least 3 or more antimicrobial agents, 64.3% of them were resistant to 4 or more antimicrobial agents.
使用ISO 10273和冷增菌法对总共300份食品样本进行了检查，其中包括180份牛奶和120份肉类产品，以检测耶尔森菌属的存在情况。耶尔森菌属的总体检出率为84份（28%）。从300份样本中的18份（6%）分离出了小肠结肠炎耶尔森菌。在样本中还检测到了其他耶尔森菌属物种，罗德耶尔森菌15份（5%）、中间耶尔森菌14份（4.7%）、假结核耶尔森菌12份（4%）、鲁氏耶尔森菌12份（4%）、莫氏耶尔森菌5份（1.7%）、贝氏耶尔森菌4份（1.3%）以及非典型耶尔森菌属4份（1.3%）。常规鉴定的小肠结肠炎耶尔森菌菌株也通过16S rRNA基因测序得到了确认。所有生物分型为1A的小肠结肠炎耶尔森菌菌株在表型毒力试验中结果均为阴性。还对84株耶尔森菌进行了毒力基因的基因型检测。除仅1株中间耶尔森菌和2株小肠结肠炎耶尔森菌被发现ystB呈阳性外，小肠结肠炎耶尔森菌和其他耶尔森菌菌株均未含有ail、ystA、yadA和virF基因。采用纸片扩散法测定了84株耶尔森菌对16种抗菌药物的耐药性。所有菌株对妥布霉素和亚胺培南敏感，而对克林霉素耐药。尽管84.5%的菌株对至少3种或更多抗菌药物耐药，但其中64.3%的菌株对4种或更多抗菌药物耐药。
