Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, 35516, Mansoura, Egypt.
Al-Azhar University, Mansoura, Egypt.
Braz J Biol. 2021 Mar-May;81(2):424-436. doi: 10.1590/1519-6984.229998.
Pathogenic Yersinia enterocolitica (Y. enterocolitica) is one of the food-borne entero-pathogen responsible for yersiniosis in humans. The purpose of this research was to survey the prevalence, virulence-associated genes, and antimicrobial resistance of Y. enterocolitica isolated from meat and meat product samples in Egypt. Forty-one (5.9%) out of 700- samples of chicken meat, beef, ground beef, and sausage were positive Y. enterocolitica with a high prevalence in chicken meat (12%). Five virulence genes (ail, inv, ystA, ystB, and yadA) were characterized among 41 Y. enterocolitica isolates with variable frequencies. Among the strains tested, the ystB gene was detected with a high percentage (78.1%), followed by inv gene (70.7%), ail gene (14.6%), ystA gene (12.2%), and yadA gene (2.4%). A high resistance rate was estimated to amoxicillin-clavulanic acid (100%), followed by cefazolin (95%), ampicillin (65.9%), and doxycycline (51.2%), whilst a high sensitivity rate was observed to gentamicin and ciprofloxacin (97.6% each). Interestingly, the multidrug resistance was specified in the 70.7% of strains and showing 13 resistance patterns. Based on nucleotide sequence analysis of the 16s rRNA gene, the phylogenetic tree showed the genetic relatedness amongst Y. enterocolitica isolates. These findings highlighted the emergence of virulent and multidrug-resistant pathogenic Y. entrocolitica in retailed meat and meat products in Egypt.
致病性肠炎耶尔森菌（Y. enterocolitica）是一种食源性肠道病原体，可导致人类耶尔森菌病。本研究旨在调查埃及肉类和肉类产品样本中分离的肠炎耶尔森菌的流行情况、毒力相关基因和抗菌药物耐药性。700 份鸡肉、牛肉、绞碎牛肉和香肠样本中，有 41 份（5.9%）为肠炎耶尔森菌阳性，鸡肉中的阳性率较高（12%）。在 41 株肠炎耶尔森菌中，有 5 种毒力基因（ail、inv、ystA、ystB 和 yadA），其出现频率不同。在所测试的菌株中，ystB 基因的检出率最高（78.1%），其次是 inv 基因（70.7%）、ail 基因（14.6%）、ystA 基因（12.2%）和 yadA 基因（2.4%）。对阿莫西林-克拉维酸的耐药率最高（100%），其次是头孢唑林（95%）、氨苄西林（65.9%）和强力霉素（51.2%），而对庆大霉素和环丙沙星的敏感性率最高（各为 97.6%）。有趣的是，70.7%的菌株表现出多重耐药性，显示出 13 种耐药模式。基于 16S rRNA 基因的核苷酸序列分析，系统发育树显示了肠炎耶尔森菌分离株之间的遗传相关性。这些发现强调了在埃及零售肉类和肉类产品中出现了毒力强和多药耐药的致病性肠炎耶尔森菌。
