Thakker Janki N, Dalwadi Pranay, Dhandhukia Pinakin C
Department of Biotechnology, P. D. Patel Institute of Applied Science, Charotar University of Science & Technology, Education Campus Changa, Changa 388421, India.
Ashok and Rita Patel Institute of Integrated Study & Research in Biotechnology and Allied Sciences, New Vallabh Vidyanagar 388 121, India.
ISRN Biotechnol. 2012 Nov 11;2013:515091. doi: 10.5402/2013/515091. eCollection 2013.
The development of reliable processes for the synthesis of gold nanoparticles is an important aspect of current nanotechnology research. Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms. However, these methods of synthesis are rather slow. In present study, rapid and extracellular synthesis of gold nanoparticles using a plant pathogenic fungus F. oxysporum f. sp. cubense JT1 (FocJT1) is reported. Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min. Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis. The particles synthesized were of 22 nm sized, capped by proteins, and posed antimicrobial activity against Pseudomonas sp.
可靠的金纳米颗粒合成方法的开发是当前纳米技术研究的一个重要方面。最近，有关于使用微生物在细胞外以及细胞内生物合成金纳米颗粒的报道。然而，这些合成方法相当缓慢。在本研究中，报道了利用植物病原真菌尖孢镰刀菌古巴专化型JT1（FocJT1）快速进行细胞外合成金纳米颗粒的方法。将FocJT1菌丝体与氯化金溶液孵育60分钟即可产生金纳米颗粒。通过紫外可见光谱、傅里叶变换红外光谱和粒度分析对金纳米颗粒进行了表征。合成的颗粒大小为22纳米，由蛋白质包覆，并对假单胞菌具有抗菌活性。
