一次性热塑性微流体破裂阀可实现芯片上试剂存储。
Single-use thermoplastic microfluidic burst valves enabling on-chip reagent storage.
作者信息
Rahmanian Omid D, DeVoe Don L
机构信息
Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Department of Bioengineering, University of Maryland, College Park, MD 20742, USA. Department of Mechanical Engineering, University of Maryland, College Park, MD 20742, USA.
出版信息
Microfluid Nanofluidics. 2015 May 1;18(5-6):1045-1053. doi: 10.1007/s10404-014-1494-8.
A simple and reliable method for fabricating single-use normally closed burst valves in thermoplastic microfluidic devices is presented, using a process flow that is readily integrated into established workflows for the fabrication of thermoplastic microfluidics. An experimental study of valve performance reveals the relationships between valve geometry and burst pressure. The technology is demonstrated in a device employing multiple valves engineered to actuate at different inlet pressures that can be generated using integrated screw pumps. On-chip storage and reconstitution of fluorescein salt sealed within defined reagent chambers are demonstrated. By taking advantage of the low gas and water permeability of cyclic olefin copolymer, the robust burst valves allow on-chip hermetic storage of reagents, making the technology well suited for the development of integrated and disposable assays for use at the point of care.
本文介绍了一种在热塑性微流控器件中制造一次性常闭爆破阀的简单可靠方法，该方法采用的工艺流程可轻松集成到热塑性微流控制造的既定工作流程中。对阀门性能的实验研究揭示了阀门几何形状与爆破压力之间的关系。该技术在一种采用多个阀门的器件中得到了演示，这些阀门设计为在不同的入口压力下启动，而这些压力可通过集成螺杆泵产生。文中展示了在限定试剂腔内密封的荧光素盐的片上储存和重构。通过利用环烯烃共聚物的低气体和水渗透性，坚固的爆破阀可实现试剂的片上密封储存，使该技术非常适合开发即时检测用的集成式一次性检测方法。
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