Liu Jia, Yi Long, Xiang Zimin, Zhong Jianfeng, Zhang Hao, Sun Tiansheng
Department of Medical School of Chinese PLA Beijing 100583, China ; Department of Institute of Orthopaedics, Chinese PLA (People's Liberation Army) Beijing Army General Hospital Dongcheng District, Nanmencang Number 5, Beijing 100700, China.
Department of Institute of Orthopaedics, Chinese PLA (People's Liberation Army) Beijing Army General Hospital Dongcheng District, Nanmencang Number 5, Beijing 100700, China.
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1237-46. eCollection 2015.
Spinal cord injury (SCI)-induced systemic inflammatory response affects multiple organs outside the spinal cord. Treatment options for such complications are lacking. We studied the potential protective effects of resveratrol on SCI-induced inflammatory damage in rat lungs. Sprague-Dawley rats were subjected to weight-drop impact at the T10 vertebral level with administration of resveratrol (100 mg/kg) or vehicle (via the intraperitoneal route) immediately after trauma. Lung injury was studied by measuring: vascular permeability-related pulmonary edema; histopathologic scores, neutrophil infiltration and concentrations of inflammatory cytokines in bronchoalveolar fluid; expression of inflammatory enzymes and sirtuin (SIRT) 1 as well as nuclear factor-kappa B (NF-κB) activity in pulmonary tissues. Resveratrol treatment significantly alleviated SCI-induced pulmonary edema as indicated by the ratio of the wet weight to dry weight of lung tissue and pulmonary permeability index. Resveratrol significantly reduced neutrophil infiltration and production of inflammatory mediators. Resveratrol treatment was accompanied by up-regulation of expression of SIRT1 and suppression of NF-κB activity in pulmonary tissues. These data suggest that resveratrol may protect the lungs from SCI-induced inflammatory damage, and could be used as a therapeutic option against pulmonary problems after SCI.
脊髓损伤（SCI）引发的全身炎症反应会影响脊髓以外的多个器官。目前缺乏针对此类并发症的治疗选择。我们研究了白藜芦醇对大鼠SCI诱导的肺部炎症损伤的潜在保护作用。对Sprague-Dawley大鼠在T10椎体水平进行重物坠落撞击，并在创伤后立即给予白藜芦醇（100 mg/kg）或溶剂（通过腹腔途径）。通过测量以下指标研究肺损伤：与血管通透性相关的肺水肿；组织病理学评分、中性粒细胞浸润以及支气管肺泡灌洗液中炎症细胞因子的浓度；肺组织中炎症酶和沉默调节蛋白（SIRT）1的表达以及核因子-κB（NF-κB）活性。白藜芦醇治疗显著减轻了SCI诱导的肺水肿，这通过肺组织湿重与干重之比和肺通透性指数得以体现。白藜芦醇显著减少了中性粒细胞浸润和炎症介质的产生。白藜芦醇治疗伴随着肺组织中SIRT1表达的上调和NF-κB活性的抑制。这些数据表明，白藜芦醇可能保护肺部免受SCI诱导的炎症损伤，并可作为治疗SCI后肺部问题的一种选择。
