Zhang Xiaotao, Hou Hongwei, Xiong Wei, Hu Qingyuan
China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou, Henan 450001, China.
J Anal Methods Chem. 2015;2015:514320. doi: 10.1155/2015/514320. Epub 2015 Apr 20.
A liquid chromatographic tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of 1-hydroxypyrene (1-OHP), 3-hydroxybenzo[a]pyrene (3-OHBaP), and 3-hydroxybenz[a]anthracene (3-OHBaA) in human urine has been developed. With the exception of 3-OHBaP at a low spiking level, the average recoveries were greater than 80%. The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke. The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine). Urinary 3-OHBaA concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 ± 4.39 ng/g creatinine, 11.01 ± 13.30 ng/g creatinine, and 9.17 ± 12.89 ng/g creatinine, respectively. Urinary 3-OHBaP concentrations in nonsmokers and smokers with 8 mg and 13 mg tar cigarettes were 1.30 ± 0.20 ng/g creatinine, 2.83 ± 1.78 ng/g creatinine, and 6.00 ± 4.44 ng/g creatinine, respectively. Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).
已开发出一种液相色谱串联质谱法（LC-MS/MS），用于同时测定人尿中的1-羟基芘（1-OHP）、3-羟基苯并[a]芘（3-OHBaP）和3-羟基苯并[a]蒽（3-OHBaA）。除了低加标水平下的3-OHBaP外，平均回收率大于80%。该方法具有良好的准确度（72.1-107.7%）和重现性（1.8-11.4%），并成功用于研究香烟烟雾中芘、苯并[a]芘和苯并[a]蒽的摄取情况。结果表明，吸烟组的尿1-OHP浓度（66.58±70.91 ng/g肌酐）高于非吸烟组（58.16±49.48 ng/g肌酐）。非吸烟者以及吸8 mg和10 mg焦油香烟的吸烟者的尿3-OHBaA浓度分别为10.98±4.39 ng/g肌酐、11.01±13.30 ng/g肌酐和9.17±12.89 ng/g肌酐。非吸烟者以及吸8 mg和13 mg焦油香烟的吸烟者的尿3-OHBaP浓度分别为1.30±0.20 ng/g肌酐、2.83±1.78 ng/g肌酐和6.00±4.44 ng/g肌酐。在加拿大强烈吸烟条件下，尿1-OHP水平与香烟烟雾中的苯并[a]芘产率呈现显著相关性（y = 3.5563x + 30.171，R (2) = 0.9916，n = 227）。
