Gruľová Daniela, Baranová Beata, Eliašová Adriana, Brun Christelle, Fejér Jozef, Kron Ivan, Campone Luca, Pagliari Stefania, Nastišin Ľuboš, Sedlák Vincent
Department of Ecology, Faculty of Humanities and Natural Sciences, University of Prešov, 17. Novembra 1, 080 01 Prešov, Slovakia.
Department of Biotechnology and Bioscience, University of Milano-Bicocca, 20126 Milan, Italy.
Plants (Basel). 2024 May 12;13(10):1333. doi: 10.3390/plants13101333.
is an invasive species in middle Europe. The mode of action of its invasiveness is still not known. Our study focuses on observation of potential allelopathic influence by the production and release of phytochemicals into its environment. Plant material was collected four times within one season (April, May, June, July 2019) at locality Lekárovce (eastern Slovakia) for comparison of differences in composition and potential allelopathy. Water extracts from collected samples were used for different biological assays. The total phenols and flavonoids were determined spectrophotometrically. The profile and content of phenolic components, including coumarins, were determined by two techniques of liquid chromatography along with in vitro evaluation of the free radical scavenging activity of extracts (DPPH, Hydroxyl, Superoxide, and FRAP). The changes in composition in extracts in different seasonal periods were evident as well as potential phytotoxic activity in some concentrations on specific model plants. The slight antioxidant activity was noted. The invasiveness of the current species could be supported by the excretion of its phytochemicals into its surroundings and by different modes of action influencing living organisms in its environment.
是中欧的一种入侵物种。其入侵的作用方式尚不清楚。我们的研究重点是观察其通过向环境中产生和释放植物化学物质而产生的潜在化感影响。在一个季节内（2019年4月、5月、6月、7月），在莱卡罗夫采（斯洛伐克东部）采集了四次植物材料，以比较其成分差异和潜在化感作用。采集样品的水提取物用于不同的生物测定。通过分光光度法测定总酚和黄酮类化合物。采用两种液相色谱技术测定酚类成分（包括香豆素）的谱图和含量，并对提取物的自由基清除活性（DPPH、羟基、超氧阴离子和FRAP）进行体外评价。不同季节提取物成分的变化明显，且在某些浓度下对特定模式植物具有潜在的植物毒性活性。还观察到了轻微的抗氧化活性。该物种的入侵性可能是通过其植物化学物质向周围环境的排泄以及通过影响其环境中生物的不同作用方式来实现的。
