Graduate Program in Neurosciences, Stanford University School of Medicine , Stanford, California 94305, USA ; Department of Molecular and Cellular Physiology, Stanford University School of Medicine , Stanford, California 94305, USA.
Department of Molecular and Cellular Physiology, Stanford University School of Medicine , Stanford, California 94305, USA ; Allen Institute for Brain Science , Seattle, Washington 98103, USA.
Sci Data. 2014 Dec 23;1:140046. doi: 10.1038/sdata.2014.46. eCollection 2014.
A major question in neuroscience is how diverse subsets of synaptic connections in neural circuits are affected by experience dependent plasticity to form the basis for behavioral learning and memory. Differences in protein expression patterns at individual synapses could constitute a key to understanding both synaptic diversity and the effects of plasticity at different synapse populations. Our approach to this question leverages the immunohistochemical multiplexing capability of array tomography (ATomo) and the columnar organization of mouse barrel cortex to create a dataset comprising high resolution volumetric images of spared and deprived cortical whisker barrels stained for over a dozen synaptic molecules each. These dataset has been made available through the Open Connectome Project for interactive online viewing, and may also be downloaded for offline analysis using web, Matlab, and other interfaces.
神经科学中的一个主要问题是，神经网络中不同的突触连接子集如何受到经验依赖可塑性的影响，从而为行为学习和记忆奠定基础。单个突触处的蛋白质表达模式的差异可能是理解突触多样性和不同突触群体可塑性影响的关键。我们解决这个问题的方法利用了原子力显微镜免疫荧光多重标记（ATomo）的免疫组织化学多重标记能力和小鼠桶状皮层的柱状组织，创建了一个数据集，其中包含了十几个突触分子染色的、受保留和剥夺影响的皮质 whisker 桶的高分辨率容积图像。该数据集已通过开放连接组计划（Open Connectome Project）提供，可进行在线交互式浏览，也可以使用网络、Matlab 和其他接口下载进行离线分析。
