Hong Bong Jin, Eryazici Ibrahim, Bleher Reiner, Thaner Ryan V, Mirkin Chad A, Nguyen SonBinh T
†Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.
‡Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, Illinois 60208-3108, United States.
J Am Chem Soc. 2015 Jul 1;137(25):8184-91. doi: 10.1021/jacs.5b03485. Epub 2015 Jun 19.
Complementary tetrahedral small molecule-DNA hybrid (SMDH) building blocks have been combined to form nucleic acid-based polymeric nanoparticles without the need for an underlying template or scaffold. The sizes of these particles can be tailored in a facile fashion by adjusting assembly conditions such as SMDH concentration, assembly time, and NaCl concentration. Notably, these novel particles can be stabilized and transformed into functionalized spherical nucleic acid (SNA) structures through the incorporation of capping DNA strands conjugated with functional groups. These results demonstrate a systematic, efficient strategy for the construction and surface functionalization of well-defined, size-tunable nucleic acid particles from readily accessible molecular building blocks. Furthermore, because these nucleic acid-based polymeric nanoparticles exhibited enhanced cellular internalization and resistance to DNase I compared to free synthetic nucleic acids, they should have a plethora of applications in diagnostics and therapeutics.
互补四面体小分子 - DNA 杂交（SMDH）构建块已被组合形成基于核酸的聚合物纳米颗粒，无需潜在的模板或支架。通过调整组装条件，如 SMDH 浓度、组装时间和 NaCl 浓度，可以轻松地定制这些颗粒的尺寸。值得注意的是，通过掺入与官能团共轭的封端 DNA 链，这些新型颗粒可以被稳定并转化为功能化的球形核酸（SNA）结构。这些结果展示了一种系统、高效的策略，用于从易于获得的分子构建块构建定义明确、尺寸可调的核酸颗粒并进行表面功能化。此外，由于这些基于核酸的聚合物纳米颗粒与游离的合成核酸相比，表现出增强的细胞内化和对 DNase I 的抗性，它们在诊断和治疗中应该有大量的应用。
