Bruckman Michael A, VanMeter Allen, Steinmetz Nicole F
Department of Biomedical Engineering, Department of Radiology, Department of Materials Science and Engineering, and Department of Macromolecular Engineering, Case Western Reserve University Schools of Medicine and Engineering , 10900 Euclid Avenue, Cleveland, Ohio 44106, United States.
ACS Biomater Sci Eng. 2015 Jan 12;1(1):13-18. doi: 10.1021/ab500059s. Epub 2014 Dec 9.
Nanomanufacturing of nanoparticles is critical for potential translation and commercialization. Continuous flow devices can alleviate this need through unceasing production of nanoparticles. Here we demonstrate the scaled-up production of spherical nanoparticles functionalized with biomedical cargos from the rod-shaped plant virus tobacco mosaic virus (TMV) using a mesofluidic, continued flow method. Production yields were increased 30-fold comparing the mesofluidic device versus batch methods. Finally, we produced MRI contrast agents of select sizes, with per particle relaxivity reaching 979,218 mM s at 60 MHz. These TMV-based spherical nanoparticle MRI contrast agents are in the top echelon of relaxivity per nanoparticle.
纳米颗粒的纳米制造对于其潜在的转化和商业化至关重要。连续流动装置可以通过不断生产纳米颗粒来满足这一需求。在此，我们展示了使用介观流体连续流动方法，从杆状植物病毒烟草花叶病毒（TMV）大规模生产负载生物医学物质的球形纳米颗粒。与分批方法相比，介观流体装置的产量提高了30倍。最后，我们制备了特定尺寸的MRI造影剂，在60 MHz时每个颗粒的弛豫率达到979,218 mM s。这些基于TMV的球形纳米颗粒MRI造影剂在每个纳米颗粒的弛豫率方面处于顶级水平。
