Priore Salvatore F, Kauffmann Andrew D, Baman Jayson R, Turner Douglas H
Department of Chemistry and Center for RNA Biology, University of Rochester, Rochester, New York 14627, United States.
Biochemistry. 2015 Jun 9;54(22):3413-5. doi: 10.1021/bi501564d. Epub 2015 May 27.
Influenza A is a negative-sense RNA virus with an eight-segment genome. Some segments encode more than one polypeptide product, but how the virus accesses alternate internal open reading frames (ORFs) is not completely understood. In segment 2, ribosomal scanning produces two internal ORFs, PB1-F2 and N40. Here, chemical mapping reveals a Mg(2+)-dependent pseudoknot structure that includes the PB1-F2 and N40 start codons. The results suggest that interactions of the ribosome with the pseudoknot may affect the level of translation for PB1-F2 and N40.
甲型流感病毒是一种具有八段基因组的负链RNA病毒。一些片段编码不止一种多肽产物，但病毒如何进入交替的内部开放阅读框（ORF）尚未完全清楚。在片段2中，核糖体扫描产生两个内部ORF，即PB1-F2和N40。在此，化学图谱揭示了一种依赖Mg(2+)的假结结构，其包含PB1-F2和N40起始密码子。结果表明，核糖体与假结的相互作用可能会影响PB1-F2和N40的翻译水平。
