Pastor Nina, Amero Carlos
Laboratorio de Dinámica de Proteínas y Ácidos Nucleicos, Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos , Cuernavaca, Mexico.
Laboratorio de Bioquímica y Resonancia Magnética Nuclear, Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos , Cuernavaca, Mexico.
Front Plant Sci. 2015 May 5;6:306. doi: 10.3389/fpls.2015.00306. eCollection 2015.
Proteins participate in information pathways in cells, both as links in the chain of signals, and as the ultimate effectors. Upon ligand binding, proteins undergo conformation and motion changes, which can be sensed by the following link in the chain of information. Nuclear magnetic resonance (NMR) spectroscopy and molecular dynamics (MD) simulations represent powerful tools for examining the time-dependent function of biological molecules. The recent advances in NMR and the availability of faster computers have opened the door to more detailed analyses of structure, dynamics, and interactions. Here we briefly describe the recent applications that allow NMR spectroscopy and MD simulations to offer unique insight into the basic motions that underlie information transfer within and between cells.
蛋白质在细胞的信息通路中发挥作用，既作为信号链中的环节，也作为最终效应器。配体结合后，蛋白质会发生构象和运动变化，这些变化可被信息链中的下一个环节感知。核磁共振（NMR）光谱学和分子动力学（MD）模拟是研究生物分子时间依赖性功能的强大工具。NMR的最新进展以及更快计算机的出现，为更详细地分析结构、动力学和相互作用打开了大门。在此，我们简要描述最近的一些应用，这些应用使NMR光谱学和MD模拟能够为细胞内和细胞间信息传递所基于的基本运动提供独特见解。
