School of Dentistry/Unesp, São Paulo State University - Unesp, Rua Humaitá, 1680, Zip code 14801-903, Araraquara, SP, Brazil(2).
Institute of Chemistry - São Paulo State University - Unesp, P.O. Box 355, Araraquara, SP 14801-970, Brazil(1); Laboratório de Química Medicinal e Medicina Regenerativa (QUIMMERA) - Centro Universitário de Araraquara (UNIARA), Araraquara, SP, Brazil.
Carbohydr Polym. 2015 Sep 5;128:41-51. doi: 10.1016/j.carbpol.2015.04.007. Epub 2015 Apr 17.
Bacterial cellulose (BC) and silk fibroin (SF) are natural biopolymers successfully applied in tissue engineering and biomedical fields. In this work nanocomposites based on BC and SF were prepared and characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). In addition, the investigation of cytocompatibility was done by MTT, XTT and Trypan Blue dye technique. Cellular adhesion and proliferation were detected additionally. The evaluation of genotoxicity was realized by micronucleus assay. In vitro tests showed that the material is non-cytotoxic or genotoxic. SEM images revealed a greater number of cells attached at the BC/SF:50% scaffold surface than the pure BC one, suggesting that the presence of fibroin improved cell attachment. This could be related to the SF amino acid sequence that acts as cell receptors facilitating cell adhesion and growth. Consequently, BC/SF:50% scaffolds configured an excellent option in bioengineering depicting its potential for tissue regeneration and cultivation of cells on nanocomposites.
细菌纤维素 (BC) 和丝素蛋白 (SF) 是成功应用于组织工程和生物医学领域的天然生物聚合物。在这项工作中，制备了基于 BC 和 SF 的纳米复合材料，并通过扫描电子显微镜 (SEM)、红外光谱 (FT-IR)、X 射线衍射 (XRD) 和热重分析 (TGA) 进行了表征。此外，通过 MTT、XTT 和台盼蓝染料技术进行了细胞相容性研究。还检测了细胞黏附和增殖。通过微核试验实现了遗传毒性的评估。体外测试表明该材料无细胞毒性或遗传毒性。SEM 图像显示，与纯 BC 相比，BC/SF:50%支架表面附着的细胞数量更多，这表明丝素的存在改善了细胞附着。这可能与丝素的氨基酸序列有关，该序列作为细胞受体，促进细胞黏附和生长。因此，BC/SF:50%支架在生物工程中是一种极好的选择，表明其在组织再生和纳米复合材料上细胞培养方面具有潜力。
