Wu Jian, Yin Rui-Xing, Guo Tao, Lin Quan-Zhen, Shen Shao-Wen, Sun Jia-Qi, Shi Guang-Yuan, Wu Jin-Zhen, Yang De-Zhai, Lin Wei-Xiong
Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
Department of Molecular Biology, Medical Scientific Research Center, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
Mol Med Rep. 2015 Sep;12(3):3476-3486. doi: 10.3892/mmr.2015.3823. Epub 2015 May 22.
Cytoplasmic poly(A) binding protein 4 (PABPC4) is an RNA-processing protein which has an important role in regulating gene expression. The association of the PABPC4 rs4660293 single nucleotide polymorphism (SNP) and serum lipid profiles has, to the best of our knowledge, not previously been studied in the Chinese population. The present study aimed to investigate the association between the PABPC4 rs4660293 SNP and several environmental factors with serum lipid levels in the Mulao and Han populations. A total of 727 individuals of Mulao nationality and 729 individuals of Han nationality were randomly selected from stratified randomized samples from a previous study by our group. Genotypes of the PABPC4 rs4660293 SNP were determined via polymerase chain reaction and restriction fragment length polymorphism analyses and subsequently confirmed by direct sequencing. Serum levels of low-density lipoprotein cholesterol (LDL-C) and apolipoprotein (Apo) B were higher in the Mulao group than those in the Han group (P<0.01 for each). The genotypic and allelic frequencies of the PABPC4 rs4660293 SNP were significantly different between males and females in the Mulao population (P<0.05 for each), while no significant difference was detected between those of males and females amongst the Han population. The frequency of the G allele was higher in Mulao males than in Mulao females (22.12 vs. 13.44%). The G allele carriers were found to have higher total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and ApoAI levels in Han females but not in Han males, and lower TC and HDL-C levels in Mulao females but not in Mulao males than those of the G allele non-carriers (P<0.05 for all). These associations were confirmed by multiple linear regression analysis (P<0.05‑0.001). Serum lipid parameters were also correlated with multiple environmental factors (P<0.05‑0.001). The PABPC4 rs4660293 SNP was associated with serum TC, HDL-C, LDL-C and ApoAI levels in these study populations; however, the association varied between the Mulao and Han populations. A gender-specific association was identified in the populations of the two ethnic groups.
细胞质聚腺苷酸结合蛋白4（PABPC4）是一种RNA加工蛋白，在调节基因表达中起重要作用。据我们所知，PABPC4 rs4660293单核苷酸多态性（SNP）与血脂谱的关联此前尚未在中国人群中进行过研究。本研究旨在探讨PABPC4 rs4660293 SNP以及几种环境因素与仫佬族和汉族人群血脂水平之间的关联。从我们团队之前一项研究的分层随机样本中随机选取了727名仫佬族个体和729名汉族个体。通过聚合酶链反应和限制性片段长度多态性分析确定PABPC4 rs4660293 SNP的基因型，随后通过直接测序进行确认。仫佬族组的血清低密度脂蛋白胆固醇（LDL-C）和载脂蛋白（Apo）B水平高于汉族组（每项P<0.01）。仫佬族人群中，PABPC4 rs4660293 SNP的基因型和等位基因频率在男性和女性之间存在显著差异（每项P<0.05），而汉族人群中男性和女性之间未检测到显著差异。仫佬族男性中G等位基因的频率高于仫佬族女性（22.12%对13.44%）。发现G等位基因携带者中，汉族女性的总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）和载脂蛋白A1（ApoAI）水平较高，而汉族男性则不然；仫佬族女性的TC和HDL-C水平低于G等位基因非携带者，而仫佬族男性则不然（所有P<0.05）。这些关联通过多元线性回归分析得到证实（P<0.05 - 0.001）。血脂参数也与多种环境因素相关（P<0.05 - 0.001）。在这些研究人群中，PABPC4 rs4660293 SNP与血清TC、HDL-C、LDL-C和ApoAI水平相关；然而，这种关联在仫佬族和汉族人群之间有所不同。在两个民族的人群中都发现了性别特异性关联。
