Gulubova Maya, Ivanova Koni, Ananiev Julian, Gerenova Julieta, Zdraveski Aleksandar, Stoyanov Hristo, Vlaykova Tatyana
Department of General and Clinical Pathology, Medical Faculty, Trakia University , Stara Zagora , Bulgaria.
Department of Endocrinology, Medical Faculty, Trakia University , Stara Zagora , Bulgaria.
Biotechnol Biotechnol Equip. 2014 May 4;28(3):508-517. doi: 10.1080/13102818.2014.909151. Epub 2014 Sep 25.
Thyroid cancer is one of the five most common cancers in the age between 20 and 50 years. Many factors including the potent angiogenic vascular endothelial growth factor (VEGF) and different dendritic cell types are known to be related to thyroid tumourogenesis. The study was performed to address the expression of VEGF and microvessel density in thyroid cancers and to evaluate the effect of VEGF expression in thyroid tumour cells on the dendritic cells. We investigated 65 patients with different types of thyroid carcinomas: papillary (PTC), oncocytic (OTC), follicular (FTC) and anaplastic (ATC), immunohistochemically with antibodies against VEGF, CD1a, CD83, S100 and CD31. Our results suggest that the expression of VEGF is significantly more often in PTC than ATC (92.3% vs. 60.0%, = 0.025). The microvessel density marked with CD31 in the tumour border of PTC was significantly higher as compared to FTC ( = 0.039), but not to ATC and OTC ( = 0.337 and 0.134). We found that CD1a- and CD83-positive cells were dispersed with variable density and in OC CD31 vessel numbers were positively correlated with CD83 dendritic cells in tumour stroma ( = 0.847, = 0.016). We did not find statistically significant associations of the survival of patients with PTC after the surgical therapy with VEGF expression and MVD. In conclusion we may state that VEGF expression in tumour cells of thyroid cancer can induce neovascularization and suppress dendritic cells.
甲状腺癌是20至50岁人群中最常见的五种癌症之一。已知许多因素，包括具有强大血管生成作用的血管内皮生长因子（VEGF）和不同类型的树突状细胞，都与甲状腺肿瘤的发生有关。本研究旨在探讨VEGF在甲状腺癌中的表达及微血管密度，并评估甲状腺肿瘤细胞中VEGF表达对树突状细胞的影响。我们对65例不同类型的甲状腺癌患者进行了研究，包括乳头状癌（PTC）、嗜酸细胞癌（OTC）、滤泡状癌（FTC）和未分化癌（ATC），采用抗VEGF、CD1a、CD83、S100和CD31抗体进行免疫组织化学检测。我们的结果表明，VEGF在PTC中的表达明显高于ATC（92.3%对60.0%，P = 0.025）。与FTC相比，PTC肿瘤边界处CD31标记的微血管密度显著更高（P = 0.039），但与ATC和OTC相比无显著差异（P = 0.337和0.134）。我们发现CD1a和CD83阳性细胞以不同密度分散分布，在OC中，肿瘤基质中CD31血管数量与CD83树突状细胞呈正相关（r = 0.847，P = 0.016）。我们未发现手术治疗后PTC患者的生存率与VEGF表达和MVD之间存在统计学显著关联。总之，我们可以说甲状腺癌肿瘤细胞中的VEGF表达可诱导新生血管形成并抑制树突状细胞。
