人淋巴细胞中的转谷氨酰胺酶活性：植物促细胞分裂剂的早期激活作用
Transglutaminase activity in human lymphocytes: early activation by phytomitogens.
作者信息
Novogrodsky A, Quittner S, Rubin A L, Stenzel K H
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1157-61. doi: 10.1073/pnas.75.3.1157.
Transglutaminase activity is present in human peripheral lymphocytes and is enhanced up to 15-fold within 10-30 min after treatment of the cells with concanavalin A. Phytohemagglutinin has a similar effect. The enzyme is not detected when intact cells are assayed; it is detected only in cell lysates. Concanavalin A enhances transglutaminase activity only when it is incubated with intact cells; concanavalin A treatment of cell lysates has no effect, alpha-Methyl-D-mannoside specifically inhibits the enhancement of transglutaminase activity in cells treated with concanavalin A results from the specific interaction of the lectin with its saccharide binding site on the cell surface, rather than by direct interaction with the enzyme itself. The increased activity of transglutaminase in cells treated with concanavalin A, as compared to unstimulated cells, is maintained under assay conditions in which saturating levels of Ca2+ are present. Transglutaminase may be involved in early cellular events leading to lymphocyte blastogenesis.
转谷氨酰胺酶活性存在于人类外周淋巴细胞中，在用伴刀豆球蛋白A处理细胞后的10 - 30分钟内，其活性增强至15倍。植物血凝素也有类似作用。在检测完整细胞时未检测到该酶；仅在细胞裂解物中检测到。伴刀豆球蛋白A仅在与完整细胞一起孵育时才增强转谷氨酰胺酶活性；用伴刀豆球蛋白A处理细胞裂解物没有效果，α - 甲基 - D - 甘露糖苷特异性抑制伴刀豆球蛋白A处理的细胞中转谷氨酰胺酶活性的增强，这是由于凝集素与其在细胞表面的糖类结合位点的特异性相互作用，而不是与酶本身的直接相互作用。与未刺激的细胞相比，用伴刀豆球蛋白A处理的细胞中转谷氨酰胺酶活性的增加在存在饱和水平Ca2 +的检测条件下得以维持。转谷氨酰胺酶可能参与导致淋巴细胞增殖的早期细胞事件。
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