Clemente Lurdes, Manageiro Vera, Jones-Dias Daniela, Correia Ivone, Themudo Patricia, Albuquerque Teresa, Geraldes Margarida, Matos Filipa, Almendra Cláudia, Ferreira Eugénia, Caniça Manuela
INIAV - National Institute for Agrarian and Veterinary Research, Microbiology and Mycology Laboratory, Estrada de Benfica, 701, 1549-011, Lisbon, Portugal.
National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisbon, Portugal; Center for the Studies of Animal Science, Institute of Agrarian and Agri-Food Sciences and Technologies, University of Oporto, Oporto, Portugal.
Res Microbiol. 2015 Sep;166(7):574-83. doi: 10.1016/j.resmic.2015.05.007. Epub 2015 Jun 5.
The impact of extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (PMAβs) of animal origin has been a public health concern. In this study, 562 Salmonella enterica and 598 Escherichia coli isolates recovered from different animal species and food products were tested for antimicrobial resistance. Detection of ESBL-, PMAβ-, plasmid-mediated quinolone resistance (PMQR)-encoding genes and integrons was performed in isolates showing non-wild-type phenotypes. Susceptibility profiles of Salmonella spp. isolates differed according to serotype and origin of the isolates. The occurrence of cefotaxime non-wild-type isolates was higher in pets than in other groups. In nine Salmonella isolates, blaCTX-M (n = 4), blaSHV-12 (n = 1), blaTEM-1 (n = 2) and blaCMY-2 (n = 2) were identified. No PMQR-encoding genes were found. In 47 E. coli isolates, blaCTX-M (n = 15), blaSHV-12 (n = 2), blaCMY-2 (n = 6), blaTEM-type (n = 28) and PMQR-encoding genes qnrB (n = 2), qnrS (n = 1) and aac(6')-Ib-cr (n = 6) were detected. To the best of our knowledge, this study is the first to describe the presence of blaCMY-2 (n = 2) and blaSHV-12 (n = 1) genes among S. enterica from broilers in Portugal. This study highlights the fact that animals may act as important reservoirs of isolates carrying ESBL-, PMAβ- and PMQR-encoding genes that might be transferred to humans through direct contact or via the food chain.
动物源超广谱β-内酰胺酶（ESBLs）和质粒介导的AmpCβ-内酰胺酶（PMAβs）的影响一直是公共卫生关注的问题。在本研究中，对从不同动物物种和食品中分离出的562株肠炎沙门氏菌和598株大肠杆菌进行了抗菌药物耐药性检测。对表现出非野生型表型的分离株进行了ESBL、PMAβ、质粒介导的喹诺酮耐药性（PMQR）编码基因和整合子的检测。沙门氏菌分离株的药敏谱因血清型和分离株来源而异。宠物中头孢噻肟非野生型分离株的发生率高于其他组。在9株沙门氏菌分离株中，鉴定出blaCTX-M（n = 4）、blaSHV-12（n = 1）、blaTEM-1（n = 2）和blaCMY-2（n = 2）。未发现PMQR编码基因。在47株大肠杆菌分离株中，检测到blaCTX-M（n = 15）、blaSHV-12（n = 2）、blaCMY-2（n = 6）、blaTEM型（n = 28）以及PMQR编码基因qnrB（n = 2）、qnrS（n = 1）和aac(6')-Ib-cr（n = 6）。据我们所知，本研究首次描述了葡萄牙肉鸡肠炎沙门氏菌中blaCMY-2（n = 2）和blaSHV-12（n = 1）基因的存在。本研究强调了动物可能作为携带ESBL、PMAβ和PMQR编码基因的分离株的重要储存库，这些基因可能通过直接接触或食物链转移给人类。
