Ogilvie A, Huschka U, Kersten W
Biochim Biophys Acta. 1979 Dec 17;565(2):293-304. doi: 10.1016/0005-2787(79)90206-5.
The dependence of protein synthesis on the intracellular content of aminoacylated tRNA has been studied in mouse ascites tumor cells deprived for various amino acids. A remarkable reduction in net protein synthesis has been found only after a drastic decrease in aminoacylation of tRNA. The quantitative correlation of protein synthesis with the degree of aminoacylation suggests that a moderate amino acid starvation primarily influences the rate of elongation at the codon concerned. These results are in contrast to the findings previously reported for HeLa cells. Some crucial steps during the determination of intracellular aminoacyl-tRNA have been investigated. The reliability of the method employed has been discussed on a theoretical basis.
在缺乏各种氨基酸的小鼠腹水肿瘤细胞中，研究了蛋白质合成对氨酰化tRNA细胞内含量的依赖性。仅在tRNA氨酰化急剧减少后，才发现净蛋白质合成显著降低。蛋白质合成与氨酰化程度的定量相关性表明，适度的氨基酸饥饿主要影响相关密码子处的延伸速率。这些结果与先前报道的关于HeLa细胞的发现形成对比。已经研究了细胞内氨酰tRNA测定过程中的一些关键步骤。从理论基础上讨论了所采用方法的可靠性。
