Parker H. Petit Institute for Bioengineering and Bioscience, George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Department of Orthopaedic Surgery, Emory University, Atlanta, GA, USA.
Cartilage. 2014 Apr;5(2):74-7. doi: 10.1177/1947603513515483.
The goal of this report is to describe the outcome of sequential particulated cartilage allograft and autologous osteochondral transfer treatments for an osteochondral lesion of the medial femoral condyle.
A 44-year-old woman was treated with a particulated juvenile articular cartilage allograft (DeNovo NT) for a chondral lesion of the knee. As a result of continued pain, she had 2 further surgeries, including an autologous osteochondral transfer system procedure and finally a unicondylar knee arthroplasty. At the final procedure, the areas of the allograft and autograft tissue were biopsied for histological evaluation. The quality of the residual cartilage tissue was assessed first by equilibrium partitioning of an ionic contrast agent via micro-computed tomography (EPIC-µCT), and then by hematoxylin and eosin, Safranin O staining, and polarized light microscopy.
Despite showing good healing at 7 months postsurgery by MRI, at 28 months post DeNovo NT tissue implantation the excised cartilage tissue was heterogeneous, with some regions of hyaline-like cartilage and some regions of fibrocartilage. The later mosaicplasty may have helped maintain hyaline-like cartilage of the DeNovo NT tissue in its vicinity.
This case report describes the cartilage repair tissue produced by DeNovo NT implantation and compares it with autologous osteochondral plug tissue.
本报告旨在描述内侧股骨髁骨软骨病变的颗粒状软骨同种异体移植物和自体软骨下骨移植治疗的结果。
一名 44 岁女性因膝关节软骨病变接受了颗粒状幼年关节软骨同种异体移植物（DeNovo NT）治疗。由于持续疼痛，她接受了另外 2 次手术，包括自体软骨下骨移植系统手术和最终的单髁膝关节置换术。在最后一次手术中，对同种异体移植物和自体移植物组织的区域进行活检以进行组织学评估。首先通过微计算机断层扫描（EPIC-µCT）的离子对比剂平衡分区法评估残余软骨组织的质量，然后通过苏木精和伊红、番红 O 染色和偏光显微镜进行评估。
尽管 MRI 显示术后 7 个月有良好的愈合，但在 DeNovo NT 组织植入后 28 个月，切除的软骨组织呈异质性，有些区域呈透明软骨样，有些区域呈纤维软骨样。随后的马赛克plasty 可能有助于维持其周围 DeNovo NT 组织的透明软骨样。
本病例报告描述了 DeNovo NT 植入后产生的软骨修复组织，并将其与自体软骨下骨栓组织进行了比较。
