Menger Katja E, James Andrew M, Cochemé Helena M, Harbour Michael E, Chouchani Edward T, Ding Shujing, Fearnley Ian M, Partridge Linda, Murphy Michael P
MRC Mitochondrial Biology Unit, Cambridge CB2 0XY, UK; Institute of Ophthalmology, University College London, London EC1V 9EL, UK.
MRC Mitochondrial Biology Unit, Cambridge CB2 0XY, UK.
Cell Rep. 2015 Jun 30;11(12):1856-65. doi: 10.1016/j.celrep.2015.05.033. Epub 2015 Jun 18.
Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT), to assess cysteine-residue redox changes in Drosophila melanogaster during aging and fasting. This approach enabled us to simultaneously identify and quantify the redox state of several hundred cysteine residues in vivo. Cysteine residues within young flies had a bimodal distribution with peaks at ∼10% and ∼85% reversibly oxidized. Surprisingly, these cysteine residues did not become more oxidized with age. In contrast, 24 hr of fasting dramatically oxidized cysteine residues that were reduced under fed conditions while also reducing cysteine residues that were initially oxidized. We conclude that fasting, but not aging, dramatically alters cysteine-residue redox status in D. melanogaster.
改变蛋白质表面半胱氨酸残基的氧化还原状态是对环境挑战的重要反应。尽管衰老和禁食会改变许多氧化还原过程，但半胱氨酸残基的作用尚不确定。为了解决这个问题，我们使用了一种氧化还原蛋白质组学技术，即氧化同位素编码亲和标签（OxICAT），来评估黑腹果蝇在衰老和禁食过程中半胱氨酸残基的氧化还原变化。这种方法使我们能够在体内同时鉴定和定量数百个半胱氨酸残基的氧化还原状态。幼蝇体内的半胱氨酸残基呈现双峰分布，峰值分别在约10%和约85%的可逆氧化状态。令人惊讶的是，这些半胱氨酸残基并不会随着年龄增长而氧化程度增加。相反，禁食24小时会使在进食条件下处于还原状态的半胱氨酸残基显著氧化，同时也会使最初处于氧化状态的半胱氨酸残基还原。我们得出结论，禁食而非衰老会显著改变黑腹果蝇中半胱氨酸残基的氧化还原状态。
