Krüttner Sebastian, Traunmüller Lisa, Dag Ugur, Jandrasits Katharina, Stepien Barbara, Iyer Nirmala, Fradkin Lee G, Noordermeer Jasprina N, Mensh Brett D, Keleman Krystyna
Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.
Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056 Basel, Switzerland.
Cell Rep. 2015 Jun 30;11(12):1953-65. doi: 10.1016/j.celrep.2015.05.037. Epub 2015 Jun 18.
To adapt to an ever-changing environment, animals consolidate some, but not all, learning experiences to long-term memory. In mammals, long-term memory consolidation often involves neural pathway reactivation hours after memory acquisition. It is not known whether this delayed-reactivation schema is common across the animal kingdom or how information is stored during the delay period. Here, we show that, during courtship suppression learning, Drosophila exhibits delayed long-term memory consolidation. We also show that the same class of dopaminergic neurons engaged earlier in memory acquisition is also both necessary and sufficient for delayed long-term memory consolidation. Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation. Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII. Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.
为了适应不断变化的环境，动物会将部分而非全部学习经历巩固为长期记忆。在哺乳动物中，长期记忆巩固通常在记忆获取数小时后涉及神经通路的重新激活。目前尚不清楚这种延迟重新激活模式在动物界是否普遍，也不清楚在延迟期信息是如何存储的。在这里，我们表明，在求偶抑制学习过程中，果蝇表现出延迟的长期记忆巩固。我们还表明，在记忆获取早期参与的同一类多巴胺能神经元对于延迟的长期记忆巩固也是必需且充分的。此外，我们提供的证据表明，在学习过程中，翻译调节因子Orb2A标记蘑菇体神经元的特定突触以便随后进行巩固。巩固涉及随后招募Orb2B以及CaMKII的活性依赖性合成。因此，我们的结果为一种神经调节的、突触受限的分子在学习动物中桥接记忆获取和长期记忆巩固的作用提供了证据。
