Rosic Nedeljka N, Braun Christoph, Kvaskoff David
School of Biological Sciences, University of Queensland, St. Lucia, QLD, 4072, Australia,
Methods Mol Biol. 2015;1308:119-29. doi: 10.1007/978-1-4939-2684-8_6.
Marine organisms use mycosporine-like amino acids (MAAs) as biological sunscreens for the protection from damaging ultraviolet (UV) radiation and the prevention of oxidative stress. MAAs have been discovered in many different marine and freshwater species including cyanobacteria, fungi, and algae, but also in animals like cnidarian and fishes. Here, we describe a general method for the isolation and characterization of MAA compounds from red algae and symbiotic dinoflagellates isolated from coral hosts. This method is also suitable for the extraction and analyses of MAAs from a range of other algal and marine biota.
海洋生物利用类菌孢素氨基酸（MAAs）作为生物防晒剂，以保护自身免受有害紫外线（UV）辐射并预防氧化应激。MAAs已在许多不同的海洋和淡水物种中被发现，包括蓝细菌、真菌和藻类，也存在于诸如刺胞动物和鱼类等动物体内。在此，我们描述了一种从红藻和从珊瑚宿主中分离出的共生甲藻中分离和鉴定MAA化合物的通用方法。该方法也适用于从一系列其他藻类和海洋生物群中提取和分析MAAs。
