德国鸢尾变种佛罗伦萨鸢尾的酚类、黄酮含量、抗胆碱酯酶及抗氧化性评估
Phenolic, flavonoid contents, anticholinesterase and antioxidant evaluation of Iris germanica var; florentina.
作者信息
Ullah Farhat, Ayaz Muhammad, Sadiq Abdul, Hussain Abid, Ahmad Sajjad, Imran Muhammad, Zeb Anwar
机构信息
a Department of Pharmacy , University of Malakand , Chakdara, Dir Lower, Khyber Pakhtoonkhwa 18000 , Pakistan.
出版信息
Nat Prod Res. 2016 Jun;30(12):1440-4. doi: 10.1080/14786419.2015.1057585. Epub 2015 Jul 13.
This study was designed to investigate antioxidant and anticholinesterase potential of Iris germanica var; florentina. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory potential of plant samples were investigated by Ellman's assay. Antioxidant activity was performed using DPPH, H2O2 and ABTS free radical scavenging assays. Total phenolics and flavonoids contents were expressed in mg GAE/g dry weight and mg RTE/g, respectively. In AChE inhibition assay, Ig.Fl, Ig.Sp and Ig.Cf fractions exhibited highest activity with IC50 values of < 0.1, 5.64 and 19 μg/mL, respectively. In BChE inhibitory assay, Ig.Fl, Ig.Sp, Ig.Cf and Ig.Cr were most active with IC50 of < 0.1, < 0.1, 31 and 78 μg/mL, respectively. In DPPH assay, Ig.Fl and Ig.Cf exhibited highest inhibition of free radicals, 80.52% (IC50 = 9 μg/mL) and 78.30% (IC50 = 8 μg/mL), respectively. In ABTS assay Ig.Cr, Ig.Cf, Ig.Fl and Ig.Sp exhibited IC50 values of < 0.1, 2, 2 and 3 μg/mL, respectively.
本研究旨在调查德国鸢尾变种佛罗伦萨鸢尾的抗氧化和抗胆碱酯酶潜力。通过埃尔曼测定法研究了植物样品对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的抑制潜力。使用DPPH、H2O2和ABTS自由基清除测定法进行抗氧化活性检测。总酚和黄酮含量分别以mg GAE/g干重和mg RTE/g表示。在AChE抑制试验中，Ig.Fl、Ig.Sp和Ig.Cf组分表现出最高活性，IC50值分别为<0.1、5.64和19μg/mL。在BChE抑制试验中，Ig.Fl、Ig.Sp、Ig.Cf和Ig.Cr最具活性，IC50分别为<0.1、<0.1、31和78μg/mL。在DPPH试验中，Ig.Fl和Ig.Cf对自由基的抑制作用最高，分别为80.52%（IC50 = 9μg/mL）和78.30%（IC50 = 8μg/mL）。在ABTS试验中，Ig.Cr、Ig.Cf、Ig.Fl和Ig.Sp的IC50值分别为<0.1、2、2和3μg/mL。
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