Fan Xiaoying, Zhang Xiannian, Wu Xinglong, Guo Hongshan, Hu Yuqiong, Tang Fuchou, Huang Yanyi
Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China.
College of Life Sciences, Peking University, Beijing, 100871, China.
Genome Biol. 2015 Jul 23;16(1):148. doi: 10.1186/s13059-015-0706-1.
Circular RNAs (circRNAs) are a new class of non-polyadenylated non-coding RNAs that may play important roles in many biological processes. Here we develop a single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) method to sequence both polyadenylated and non-polyadenylated RNAs from individual cells. This method exhibits robust sensitivity, precision and accuracy. We discover 2891 circRNAs and 913 novel linear transcripts in mouse preimplantation embryos and further analyze the abundance of circRNAs along development, the function of enriched genes, and sequence features of circRNAs. Our work is key to deciphering regulation mechanisms of circRNAs during mammalian early embryonic development.
环状RNA（circRNAs）是一类新的非多聚腺苷酸化的非编码RNA，可能在许多生物学过程中发挥重要作用。在此，我们开发了一种单细胞通用的非多聚腺苷酸化RNA测序（SUPeR-seq）方法，用于对单个细胞中的多聚腺苷酸化和非多聚腺苷酸化RNA进行测序。该方法具有强大的灵敏度、精密度和准确性。我们在小鼠植入前胚胎中发现了2891个环状RNA和913个新的线性转录本，并进一步分析了环状RNA在发育过程中的丰度、富集基因的功能以及环状RNA的序列特征。我们的工作对于破译哺乳动物早期胚胎发育过程中环状RNA的调控机制至关重要。
