Boellner Stefanie, Becker Karl-Friedrich
a Institute of Pathology, Technische Universität München, Trogerstraße 18, 81675 München, Germany.
Expert Rev Mol Diagn. 2015;15(10):1277-92. doi: 10.1586/14737159.2015.1070098. Epub 2015 Jul 27.
Differences in protein levels and the activation of signaling pathways have been extensively studied in tumor tissues, but the implementation of protein profiling methods in routine hospital workflows lags far behind that of nucleic acid-based approaches. In this review, major technologies that are currently used for measuring protein abundances in human tissues are highlighted, and for each method several examples are provided. We differentiate between extract-based and section-based methods that are each further divided into targeted- and discovery-based approaches (i.e., when the proteins to be analyzed are known or for finding promising new biomarker candidates, respectively). Current problems in protein profiling are addressed and ways in which protein profiling can successfully be implemented in routine clinical workflows are shown.
蛋白质水平差异和信号通路激活已在肿瘤组织中得到广泛研究，但蛋白质分析方法在医院常规工作流程中的应用却远远落后于基于核酸的方法。在本综述中，重点介绍了目前用于测量人体组织中蛋白质丰度的主要技术，并为每种方法提供了几个示例。我们区分了基于提取物和基于切片的方法，每种方法又进一步分为靶向和发现型方法（即分别针对已知要分析的蛋白质或寻找有前景的新生物标志物候选物）。文中讨论了蛋白质分析当前存在的问题，并展示了蛋白质分析能够成功应用于常规临床工作流程的方式。
