Yoo Sung J, Sunwoo Sun Young, Ko Seong Sik, Je Sang H, Lee Dong Uk, Lyoo Young S
Department of Pathology, College of Veterinary Medicine, Konkuk University, 120 Neung-dong Street, Gwangjin-gu, Seoul, 143-701 South Korea.
Department of Pathology, College of Veterinary Medicine, Konkuk University, 120 Neung-dong Street, Gwangjin-gu, Seoul, 143-701 South Korea ; Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506 USA.
Springerplus. 2015 Jul 24;4:370. doi: 10.1186/s40064-015-1155-8. eCollection 2015.
Porcine bocavirus is classified within the genus Bocaparvovirus, family Parvoviridae. Unlike other parvoviruses, the members of genus Bocaparvovirus (bocaparvoviruses) encode an additional open reading frame (NP1). Many strains of PBoVs have been identified in domestic pigs and recognized as a potential emerging pathogen causing respiratory and gastrointestinal disease.
A new strain of porcine bocavirus (PBoV) that harbored the shortest NP1 gene among all currently characterized PBoVs (provisionally named as 'PBoV-KU14') was detected in domestic pigs. Almost the complete genome sequence was obtained, approximately 4,630 nucleotides in lengths with putative NS1, NP1, and VP1/2 genes of 1,908, 600, 1,851 bp, respectively. Phylogenetic and comparative analysis was performed using protein and nucleotide sequences. It was revealed that PBoV-KU14 belongs to the genus Bocaparvovirus and species Ungulate bocaparvovirus 4. However, phylogenetic incongruence was observed among species classifications based on the NS1, NP1 and VP1/2 proteins, which indicates a probability of crossover recombination. Conserved protein domains unique for genus Bocaparvovirus in NP1, VP1 protein were also detected.
NP1 gene truncation supposed to be caused by cross over recombination was detected in a new strain of PBoV (PBoV-KU14). Considering high rates of substitution and recombination in parvovirus, periodic surveillance study to monitor genomic variation and find new strainsof PBoVs seems to be needed.
猪博卡病毒属于细小病毒科博卡细小病毒属。与其他细小病毒不同，博卡细小病毒属的成员编码一个额外的开放阅读框（NP1）。在家猪中已鉴定出许多猪博卡病毒株，并被认为是一种可能引起呼吸道和胃肠道疾病的新兴病原体。
在家猪中检测到一种新的猪博卡病毒（PBoV）株，其NP1基因是目前所有已鉴定的PBoV株中最短的（暂命名为“PBoV-KU14”）。获得了几乎完整的基因组序列，长度约为4630个核苷酸，推测的NS1、NP1和VP1/2基因分别为1908、600和1851bp。使用蛋白质和核苷酸序列进行了系统发育和比较分析。结果表明，PBoV-KU14属于博卡细小病毒属和有蹄类博卡细小病毒4种。然而，基于NS1、NP1和VP1/2蛋白的物种分类中观察到系统发育不一致，这表明存在交叉重组的可能性。在NP1、VP1蛋白中也检测到了博卡细小病毒属特有的保守蛋白结构域。
在一株新的PBoV（PBoV-KU14）中检测到NP1基因截断，推测是由交叉重组引起的。考虑到细小病毒的高替换率和重组率，似乎需要定期进行监测研究，以监测基因组变异并发现新的PBoV株。
