采用高效液相色谱-二极管阵列检测器对韩国人参（Panax ginseng）产品中的麦芽酚进行定量分析。

Quantification of maltol in Korean ginseng (Panax ginseng) products by high-performance liquid chromatography-diode array detector.

作者信息

Jeong Hyun Cheol, Hong Hee-Do, Kim Young-Chan, Rhee Young Kyoung, Choi Sang Yoon, Kim Kyung-Tack, Kim Sung Soo, Lee Young-Chul, Cho Chang-Won

机构信息

Ginseng Research Center, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea.

出版信息

Pharmacogn Mag. 2015 Jul-Sep;11(43):657-64. doi: 10.4103/0973-1296.160452.

DOI:10.4103/0973-1296.160452

PMID:26246746

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4522857/

Abstract

BACKGROUND

Maltol, as a type of phenolic compounds, is produced by the browning reaction during the high-temperature treatment of ginseng. Thus, maltol can be used as a marker for the quality control of various ginseng products manufactured by high-temperature treatment including red ginseng. For the quantification of maltol in Korean ginseng products, an effective high-performance liquid chromatography-diode array detector (HPLC-DAD) method was developed.

MATERIALS AND METHODS

The HPLC-DAD method for maltol quantification coupled with a liquid-liquid extraction (LLE) method was developed and validated in terms of linearity, precision, and accuracy. An HPLC separation was performed on a C18 column.

RESULTS

The LLE methods and HPLC running conditions for maltol quantification were optimized. The calibration curve of the maltol exhibited good linearity (R (2) = 1.00). The limit of detection value of maltol was 0.26 μg/mL, and the limit of quantification value was 0.79 μg/mL. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were <1.27% and 0.61%, respectively. The results of the recovery test were 101.35-101.75% with an RSD value of 0.21-1.65%. The developed method was applied successfully to quantify the maltol in three ginseng products manufactured by different methods.

CONCLUSION

The results of validation demonstrated that the proposed HPLC-DAD method was useful for the quantification of maltol in various ginseng products.

摘要

背景

麦芽酚作为一种酚类化合物，是人参在高温处理过程中通过褐变反应产生的。因此，麦芽酚可作为包括红参在内的各种经高温处理的人参制品质量控制的标志物。为了定量测定人参制品中的麦芽酚，开发了一种有效的高效液相色谱 - 二极管阵列检测器（HPLC - DAD）方法。

材料与方法

开发了用于麦芽酚定量的HPLC - DAD方法，并结合液 - 液萃取（LLE）方法，在线性、精密度和准确度方面进行了验证。在C18柱上进行HPLC分离。

结果

优化了麦芽酚定量的LLE方法和HPLC运行条件。麦芽酚的校准曲线显示出良好的线性（R（2） = 1.00）。麦芽酚的检测限为0.26μg/mL，定量限为0.79μg/mL。日内和日间实验数据的相对标准偏差（RSD）分别<1.27%和0.61%。回收率测试结果为101.35 - 101.75%，RSD值为0.21 - 1.65%。所开发的方法成功应用于定量三种不同方法生产的人参制品中的麦芽酚。