与富血小板血浆相比，富血小板纤维蛋白可促进大鼠跟腱的加速愈合。
Platelet-Rich Fibrin Promotes an Accelerated Healing of Achilles Tendon When Compared to Platelet-Rich Plasma in Rat.
作者信息
Dietrich Franciele, L Duré Gustavo, P Klein Caroline, F Bampi Vinícius, V Padoin Alexandre, D Silva Vinícius, Braga-Silva Jefferson
机构信息
Laboratory of Medical Abilities and Surgical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil;
Service of Hand Surgery and Reconstructive Microsurgery, São Lucas Hospital, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil;
出版信息
World J Plast Surg. 2015 Jul;4(2):101-9.
BACKGROUND
Autologous platelet concentrate has been used to improve the function and regeneration of injured tissues. Tendinopathies are common in clinical practice, although long-term treatment is required. On the basis of lead time, we compared the effect of using platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in repairing rat Achilles tendon.
METHODS
The effectiveness of using PRP and PRF was evaluated after 14 and 28 postoperative days by histological analysis. The quantification of collagen types I and III was performed by Sirius red staining. Qualitatively, the data were verified with hematoxylin-eosin (H&E) staining.
RESULTS
In Sirius red staining, no significant treatment differences were found between groups. Statistical difference was observed only between PRP (37.2% collagen) and the control group (16.2%) 14 days after treatment. Intra-groups compared twice showed a difference for collagen I (27.8% and 47.7%) and III (66.9% and 46.0%) in the PRF group. The control group showed differences only in collagen I (14.2% and 40.9%) and no other finding was observed in the PRP group. In H&E staining, PRF showed a better cellular organization when compared to the other groups at 28 days.
CONCLUSION
Our study suggests that PRF promotes accelerated regeneration of the Achilles tendon in rats, offering promising prospects for future clinical use.
背景
自体血小板浓缩物已被用于改善受损组织的功能和再生。肌腱病在临床实践中很常见，尽管需要长期治疗。基于提前期，我们比较了使用富血小板血浆（PRP）和富血小板纤维蛋白（PRF）修复大鼠跟腱的效果。
方法
术后14天和28天通过组织学分析评估使用PRP和PRF的有效性。通过天狼星红染色对I型和III型胶原进行定量分析。定性方面，数据用苏木精-伊红（H&E）染色进行验证。
结果
在天狼星红染色中，各组之间未发现显著的治疗差异。仅在治疗后14天，PRP组（胶原含量为37.2%）与对照组（胶原含量为16.2%）之间观察到统计学差异。PRF组内两次比较显示I型胶原（分别为27.8%和47.7%）和III型胶原（分别为66.9%和46.0%）存在差异。对照组仅在I型胶原方面有差异（分别为14.2%和40.9%），PRP组未观察到其他差异。在H&E染色中，与其他组相比，28天时PRF显示出更好的细胞组织。
结论
我们的研究表明，PRF可促进大鼠跟腱的加速再生，为未来临床应用提供了有前景的前景。
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