Sung Hye-Ri, Lee Jin-Man, Kim Mibang, Yun Bo-Ram, Shin Kee-Sun
Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
Department of Food Science and Technology and Basic Science Institute, Hoseo University, Asan 336-795, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Nov;65(11):4140-4145. doi: 10.1099/ijsem.0.000543. Epub 2015 Aug 25.
A Gram-stain-negative, non-motile, aerobic bacterium, strain 13-93-B1T, was isolated from seawater off Jeju Island, Republic of Korea, and was subjected to polyphasic taxonomic study. Cells formed ivory colonies and were ovoid to rod-shaped. The strain was catalase-positive, oxidase-negative and grew optimally at 30 °C, in the presence of 1-2 % (w/v) NaCl and at pH 7.0-7.5. It did not synthesize bacteriochlorophyll a. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain 13-93-B1T clustered with the type strain Donghicola eburneus SW-277T (97.0 % 16S rRNA gene sequence similarity). DNA-DNA hybridization between strain 13-93-B1T and D. eburneus KCTC 12735T was 33.1 ± 1.4 % (35.2 ± 2.8 % in a reciprocal experiment). The predominant cellular fatty acid was summed feature 8 (C18 : 1ω7c/C18 : 1ω6c; 76.9 %). The major respiratory quinone was ubiquinone Q-10 and polar lipids detected in strain 13-93-B1T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid and unidentified lipids. The DNA G+C content of strain 13-93-B1T was 60.4 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain 13-93-B1T is considered to represent a novel species of the genus Donghicola, for which the name Donghicola tyrosinivorans sp. nov. is proposed. The type strain is 13-93-B1T ( = DSM 100212T = KCTC 42571T).
从韩国济州岛附近海域分离出一株革兰氏染色阴性、无运动性、需氧的细菌，菌株13 - 93 - B1T，并对其进行了多相分类研究。细胞形成象牙色菌落，呈卵形至杆状。该菌株过氧化氢酶阳性、氧化酶阴性，在30 °C、1 - 2 %（w/v）NaCl存在及pH 7.0 - 7.5条件下生长最佳。它不合成细菌叶绿素a。基于16S rRNA基因序列构建的邻接法、最大似然法和最大简约法系统发育树显示，菌株13 - 93 - B1T与模式菌株白东河菌SW - 277T聚类（16S rRNA基因序列相似性为97.0 %）。菌株13 - 93 - B1T与白东河菌KCTC 12735T之间的DNA - DNA杂交率为33.1 ± 1.4 %（反向实验中为35.2 ± 2.8 %）。主要细胞脂肪酸为总和特征8（C18 : 1ω7c/C18 : 1ω6c；76.9 %）。主要呼吸醌为泛醌Q - 10，在菌株13 - 93 - B1T中检测到的极性脂质有磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、一种未鉴定的氨基脂质和未鉴定的脂质。菌株13 - 93 - B1T的DNA G + C含量为60.4 mol%。根据所提供的表型、化学分类和系统发育数据，菌株13 - 93 - B1T被认为代表了东河菌属的一个新物种，为此提出新名称酪氨酸东河菌（Donghicola tyrosinivorans sp. nov.）。模式菌株为13 - 93 - B1T（ = DSM 100212T = KCTC 42571T）。
