School of Chemical Engineering and Australian Centre for Nanomedicine (ACN), The University of New South Wales (UNSW Sydney), Sydney, NSW 2052, Australia.
Graduate School of Biomedical Engineering, The University of New South Wales (UNSW Sydney), Sydney, NSW 2052, Australia.
Chem Asian J. 2022 Mar 1;17(5):e202101399. doi: 10.1002/asia.202101399. Epub 2022 Jan 27.
Hydrogen sulfide (H S) is a gasotransmitter known to regulate physiological and pathological processes. Abnormal H S levels have been associated with a range of conditions, including Parkinson's and Alzheimer's diseases, cardiovascular and renal diseases, bacterial and viral infections, as well as cancer. Therefore, fast and sensitive H S detection is of significant clinical importance. Fluorescent H S probes hold great potential among the currently developed detection methods because of their high sensitivity, selectivity, and biocompatibility. However, many proposed probes do not provide a gold standard for proper use and selection. Consequently, issues arise when applying the probes in different conditions. Therefore, we systematically evaluated four commercially available probes (WSP-1, WSP-5, CAY, and P3), considering their detection range, sensitivity, selectivity, and performance in different environments. Furthermore, their capacity for endogenous H S imaging in live cells was demonstrated.
硫化氢（H₂S）是一种已知能调节生理和病理过程的气体信号分子。异常的 H₂S 水平与一系列疾病有关，包括帕金森病和阿尔茨海默病、心血管和肾脏疾病、细菌和病毒感染以及癌症。因此，快速灵敏的 H₂S 检测具有重要的临床意义。在目前开发的检测方法中，荧光 H₂S 探针因其高灵敏度、选择性和生物相容性而具有很大的潜力。然而，许多提出的探针并没有为正确使用和选择提供一个黄金标准。因此，当在不同条件下应用探针时会出现问题。因此，我们系统地评估了四种市售探针（WSP-1、WSP-5、CAY 和 P3），考虑了它们的检测范围、灵敏度、选择性以及在不同环境下的性能。此外，还证明了它们在活细胞内内源 H₂S 成像的能力。
