Department of Biochemistry, University of Alberta, 561 Medical Sciences Building, Edmonton, AB, T6G 2H7, Canada.
J Ind Microbiol Biotechnol. 2018 Jan;45(1):1-14. doi: 10.1007/s10295-017-1985-1. Epub 2017 Oct 26.
The oleaginous yeast Lipomyces starkeyi was engineered for the production of long-chain fatty alcohols by expressing a fatty acyl-CoA reductase, mFAR1, from Mus musculus. The optimal conditions for production of fatty alcohols by this strain were investigated. Increased carbon-to-nitrogen ratios led to efficient C16 and C18 fatty alcohol production from glucose, xylose and glycerol. Batch cultivation resulted in a titer of 1.7 g/L fatty alcohol from glucose which represents a yield of 28 mg of fatty alcohols per gram of glucose. This relatively high level of production with minimal genetic modification indicates that L. starkeyi may be an excellent host for the bioconversion of carbon-rich waste streams, particularly lignocellulosic waste, to C16 and C18 fatty alcohols.
产油酵母史氏油脂酵母经工程改造后，可通过表达来自小家鼠的脂肪酸酰基辅酶 A 还原酶 mFAR1 来生产长链脂肪醇。本研究对该菌株生产脂肪醇的最佳条件进行了探讨。提高碳氮比有利于该菌株利用葡萄糖、木糖和甘油高效生产 C16 和 C18 脂肪醇。分批培养可使该菌株从葡萄糖中获得 1.7 g/L 的脂肪醇，葡萄糖得率为 28 mg/g。这种相对较高的产量和最小的遗传修饰表明，史氏油脂酵母可能是将富含碳的废物流（特别是木质纤维素废物）生物转化为 C16 和 C18 脂肪醇的理想宿主。
