使用水通道蛋白-0的二维晶体评估K2电子计数相机的超分辨率性能。
Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0.
作者信息
Chiu Po-Lin, Li Xueming, Li Zongli, Beckett Brian, Brilot Axel F, Grigorieff Nikolaus, Agard David A, Cheng Yifan, Walz Thomas
机构信息
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
The Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
出版信息
J Struct Biol. 2015 Nov;192(2):163-73. doi: 10.1016/j.jsb.2015.08.015. Epub 2015 Aug 28.
Abstract
The K2 Summit camera was initially the only commercially available direct electron detection camera that was optimized for high-speed counting of primary electrons and was also the only one that implemented centroiding so that the resolution of the camera can be extended beyond the Nyquist limit set by the physical pixel size. In this study, we used well-characterized two-dimensional crystals of the membrane protein aquaporin-0 to characterize the performance of the camera below and beyond the physical Nyquist limit and to measure the influence of electron dose rate on image amplitudes and phases.
摘要
K2 Summit相机最初是唯一一款市售的直接电子检测相机，它针对一次电子的高速计数进行了优化，也是唯一一款采用重心法的相机，这样相机的分辨率就能扩展到超出由物理像素大小设定的奈奎斯特极限。在本研究中，我们使用了特征明确的膜蛋白水通道蛋白-0的二维晶体，以表征该相机在物理奈奎斯特极限以下和以上的性能，并测量电子剂量率对图像幅度和相位的影响。
相似文献
1
Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0.使用水通道蛋白-0的二维晶体评估K2电子计数相机的超分辨率性能。
J Struct Biol. 2015 Nov;192(2):163-73. doi: 10.1016/j.jsb.2015.08.015. Epub 2015 Aug 28.
2
Electron crystallography and aquaporins.电子晶体学与水通道蛋白
Methods Enzymol. 2010;483:91-119. doi: 10.1016/S0076-6879(10)83005-8.
3
Image processing techniques for high-resolution structure determination from badly ordered 2D crystals.高分辨率结构测定中用于严重无序二维晶体的图像处理技术。
J Struct Biol. 2018 Aug;203(2):120-134. doi: 10.1016/j.jsb.2018.03.013. Epub 2018 Apr 22.
4
Influence of electron dose rate on electron counting images recorded with the K2 camera.电子剂量率对 K2 相机记录的电子计数图像的影响。
J Struct Biol. 2013 Nov;184(2):251-60. doi: 10.1016/j.jsb.2013.08.005. Epub 2013 Aug 20.
5
Aquaporin-0 membrane junctions reveal the structure of a closed water pore.水通道蛋白-0膜连接揭示了封闭水孔的结构。
Nature. 2004 May 13;429(6988):193-7. doi: 10.1038/nature02503.
6
Effect of lipid head groups on double-layered two-dimensional crystals formed by aquaporin-0.脂质头部基团对水通道蛋白0形成的双层二维晶体的影响。
PLoS One. 2015 Jan 30;10(1):e0117371. doi: 10.1371/journal.pone.0117371. eCollection 2015.
7
Cryo-electron microscopy of membrane proteins.膜蛋白的冷冻电子显微镜技术
Methods Mol Biol. 2014;1117:325-41. doi: 10.1007/978-1-62703-776-1_15.
8
Merging of image data in electron crystallography.电子晶体学中图像数据的合并
Methods Mol Biol. 2013;955:195-209. doi: 10.1007/978-1-62703-176-9_11.
9
Improved specimen preparation for cryo-electron microscopy using a symmetric carbon sandwich technique.使用对称碳三明治技术改进冷冻电子显微镜的样本制备方法。
J Struct Biol. 2004 Jun;146(3):325-33. doi: 10.1016/j.jsb.2004.01.012.
10
Structural models of the supramolecular organization of AQP0 and connexons in junctional microdomains.水通道蛋白0（AQP0）和连接子在连接微区的超分子组织的结构模型。
J Struct Biol. 2007 Dec;160(3):385-94. doi: 10.1016/j.jsb.2007.07.009. Epub 2007 Jul 28.
引用本文的文献
1
Structure and dynamics of cholesterol-mediated aquaporin-0 arrays and implications for lipid rafts.胆固醇介导的水通道蛋白 0 阵列的结构和动力学及其对脂筏的影响。
Elife. 2024 Sep 2;12:RP90851. doi: 10.7554/eLife.90851.
2
Molecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria.绿色硫细菌光合超复合体的分子不对称性。
Nat Commun. 2022 Oct 3;13(1):5824. doi: 10.1038/s41467-022-33505-4.
3
Probing Structural Perturbation of Biomolecules by Extracting Cryo-EM Data Heterogeneity.探究生物分子结构扰动的方法：从低温电子显微镜数据异质性中提取信息。
Biomolecules. 2022 Apr 24;12(5):628. doi: 10.3390/biom12050628.
4
High-speed high-resolution data collection on a 200 keV cryo-TEM.在200 keV低温透射电子显微镜上进行高速高分辨率数据采集。
IUCrJ. 2022 Jan 29;9(Pt 2):243-252. doi: 10.1107/S2052252522000069. eCollection 2022 Mar 1.
5
Experimental evaluation of super-resolution imaging and magnification choice in single-particle cryo-EM.单颗粒冷冻电镜中超高分辨率成像及放大倍数选择的实验评估
J Struct Biol X. 2021 Mar 13;5:100047. doi: 10.1016/j.yjsbx.2021.100047. eCollection 2021.
6
Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid-Protein Interaction.用于膜蛋白结构和脂-蛋白相互作用电子晶体学研究的样品制备和数据收集。
Methods Mol Biol. 2021;2215:247-265. doi: 10.1007/978-1-0716-0966-8_11.
7
Electron-event representation data enable efficient cryoEM file storage with full preservation of spatial and temporal resolution.电子事件表示数据能够实现高效的冷冻电镜文件存储，同时完全保留空间和时间分辨率。
IUCrJ. 2020 Aug 7;7(Pt 5):860-869. doi: 10.1107/S205225252000929X. eCollection 2020 Sep 1.
8
What Could Go Wrong? A Practical Guide to Single-Particle Cryo-EM: From Biochemistry to Atomic Models.可能会出什么问题？单颗粒冷冻电镜实用指南：从生物化学到原子模型
J Chem Inf Model. 2020 May 26;60(5):2458-2469. doi: 10.1021/acs.jcim.9b01178. Epub 2020 Mar 9.
9
Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant.亚 2 埃瓦尔德曲率校正的 AAV2 衣壳变体结构。
Nat Commun. 2018 Sep 7;9(1):3628. doi: 10.1038/s41467-018-06076-6.
10
Biological Applications at the Cutting Edge of Cryo-Electron Microscopy.冷冻电子显微镜前沿的生物学应用
Microsc Microanal. 2018 Aug;24(4):406-419. doi: 10.1017/S1431927618012382.
本文引用的文献
1
2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor.与一种细胞渗透性抑制剂结合的β-半乳糖苷酶的2.2埃分辨率冷冻电镜结构。
Science. 2015 Jun 5;348(6239):1147-51. doi: 10.1126/science.aab1576. Epub 2015 May 7.
2
Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy.用于低剂量电子显微镜的三种直接电子探测器在300keV下的最佳性能比较。
Ultramicroscopy. 2014 Dec;147:156-63. doi: 10.1016/j.ultramic.2014.08.002. Epub 2014 Aug 9.
3
Structure of the yeast mitochondrial large ribosomal subunit.酵母线粒体大亚基的结构。
Science. 2014 Mar 28;343(6178):1485-1489. doi: 10.1126/science.1249410.
4
Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector.使用直接电子探测器通过电子冷冻显微镜技术得到的F420还原型[NiFe]氢化酶的原子模型。
Elife. 2014 Feb 25;3:e01963. doi: 10.7554/eLife.01963.
5
Structure of the TRPV1 ion channel determined by electron cryo-microscopy.电子冷冻显微镜解析 TRPV1 离子通道结构。
Nature. 2013 Dec 5;504(7478):107-12. doi: 10.1038/nature12822.
6
Quantitative characterization of electron detectors for transmission electron microscopy.用于透射电子显微镜的电子探测器的定量表征。
J Struct Biol. 2013 Dec;184(3):385-93. doi: 10.1016/j.jsb.2013.10.016. Epub 2013 Nov 1.
7
Maximizing the potential of electron cryomicroscopy data collected using direct detectors.最大限度地发挥使用直接探测器收集的电子冷冻显微镜数据的潜力。
J Struct Biol. 2013 Nov;184(2):193-202. doi: 10.1016/j.jsb.2013.09.003. Epub 2013 Sep 12.
8
Influence of electron dose rate on electron counting images recorded with the K2 camera.电子剂量率对 K2 相机记录的电子计数图像的影响。
J Struct Biol. 2013 Nov;184(2):251-60. doi: 10.1016/j.jsb.2013.08.005. Epub 2013 Aug 20.
9
Characterization of an early-stage fusion intermediate of Sindbis virus using cryoelectron microscopy.利用冷冻电镜技术对辛德毕斯病毒融合早期中间体进行特性分析。
Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13362-7. doi: 10.1073/pnas.1301911110. Epub 2013 Jul 29.
10
Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM.电子计数和束流诱导运动校正可实现近原子分辨率的单颗粒冷冻电镜。
Nat Methods. 2013 Jun;10(6):584-90. doi: 10.1038/nmeth.2472. Epub 2013 May 5.
Zotero 插件