Faculty of Para-Veterinary Medicine, University of Ilam, Ilam, Iran.
Master of Sciences of Histology, Faculty of Veterinary Medicine, University of Shahid Chamran Ahwaz, Ahwaz, Iran.
J Environ Health Sci Eng. 2015 Aug 29;13:62. doi: 10.1186/s40201-015-0216-9. eCollection 2015.
Regarding fast development of the nanotechnology and the probably of it's side effects on aquatic body organs, this study investigate the effects of nanosilver administration on histology of gill, kidney and biochemical parameters in common carp. The silver nanoparticles were synthesized in a one-step reduction process in an aqueous solution. 60 O. mykiss were obtained from a local commercial hatchery. Fish were divided randomly into four groups. Control group was kept in dechlorinated tap water without any add-on material. Experimental groups were exposed to concentration of 3, 300 and 1000 mg/L of nanosilver solution for eight weeks, respectively. Biochemical analyses of sera, histological alterations of the gill and kidney tissue were done. Aneurism in the secondary lamellae and hyperplasia of epithelium in gills, adhesion of the gill lamellae, inducing hyaline cast formation, significant decreasing in the glomerular diameter and formation of intra cytoplasmic vacuoles in the various urinary tubules were seen in experimental groups. The serum levels of total protein was decreased significantly (P < 0.05) by increasing nanosilver concentration but ALP, LDH, AST and ALT increased significantly (P < 0.05). It is concluded that nanosilver induces gill and kidney damages and changes the biochemical parameters of O. mykiss juveniles in different concentrations.
鉴于纳米技术的快速发展及其可能对水生生物器官产生的副作用，本研究调查了纳米银给药对鲤鱼鳃、肾脏组织学和生化参数的影响。银纳米粒子通过一步还原法在水溶液中合成。从当地一家商业孵化场获得 60 尾 O. mykiss。将鱼随机分为四组。对照组保存在不含任何添加剂的去氯自来水。实验组分别暴露于 3、300 和 1000 mg/L 的纳米银溶液中 8 周。对血清进行生化分析，对鳃和肾脏组织的组织学变化进行了分析。在实验组中观察到次级鳃瓣的动脉瘤和上皮细胞增生、鳃瓣粘连、玻璃样 casts 形成、肾小球直径显著减小以及各种肾小管内的细胞内空泡形成。随着纳米银浓度的增加，血清总蛋白水平显著降低（P < 0.05），但 ALP、LDH、AST 和 ALT 显著升高（P < 0.05）。结论：纳米银诱导鲤鱼鳃和肾脏损伤，并改变不同浓度下 O. mykiss 幼鱼的生化参数。
