用澳大利亚库蚊（蚊子）匀浆处理的蚊子细胞系C6/36中管状聚集体的电子显微镜检测。

Electron microscope detection of tubular aggregates in the mosquito cell line C6/36 treated with Culex australicus (mosquito) homogenate.

作者信息

Marshall John A, Lynch Stacey E, Crowder Jodie C, Catton Michael G, Fegan Mark, Bruggink Leesa D

机构信息

Victorian Infectious Diseases Reference Laboratory, Melbourne, Victoria, Australia

Microbial Sciences, Pests and Diseases, Biosciences Research Division, Agricultural Productivity, Department of Economic Development, Jobs, Transport and Resources, AgriBio Centre, La Trobe University Campus, Bundoora, Victoria, Australia.

出版信息

Microscopy (Oxf). 2015 Dec;64(6):445-8. doi: 10.1093/jmicro/dfv051. Epub 2015 Aug 30.

DOI:10.1093/jmicro/dfv051

PMID:26323477

Abstract

'Tubular aggregates' are morphologically distinct cytoplasmic structures that have been linked to a variety of pathological conditions. This report documents the presence of tubular aggregates in an insect cell line (C6/36 cells derived from Aedes albopictus) following inoculation of the cells with material derived from cell culture passaged homogenized Culex australicus mosquitoes. The tubular aggregates were detected in ∼2% of treated cells and had three morphological forms that were termed primary, secondary and tertiary, with progressively greater levels of structural complexity. The findings indicate that tubular aggregates can be induced in an insect cell culture system by an unidentified agent present in some mosquitoes.

摘要

“管状聚集体”是形态上独特的细胞质结构，与多种病理状况相关。本报告记录了在用来自经细胞培养传代的匀浆澳大利亚库蚊的材料接种昆虫细胞系（源自白纹伊蚊的C6/36细胞）后，管状聚集体的存在情况。在约2%的处理细胞中检测到了管状聚集体，其有三种形态形式，分别称为初级、次级和三级，结构复杂性水平逐渐增加。这些发现表明，某些蚊子中存在的一种未知因子可在昆虫细胞培养系统中诱导管状聚集体的形成。