Ren Na, Gao Guiju, Sun Yue, Zhang Ling, Wang Huizhu, Hua Wenhao, Wan Kanglin, Li Xingwang
The National Clinical Key Department of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China.
Department of Infectious Diseases, First Hospital of Tsinghua University, Beijing 100016, P.R. China.
Mol Med Rep. 2015 Nov;12(5):6561-7. doi: 10.3892/mmr.2015.4262. Epub 2015 Aug 28.
Tuberculosis (TB) infections, caused by multidrug‑resistant Mycobacterium tuberculosis (MDR MTB), remain a significant public health concern worldwide. The regulatory mechanisms underlying the emergence of MDR MTB strains remain to be fully elucidated, and further investigation is required in order to develop better strategies for TB control. The present study investigated the expression profile of microRNA (miRNA) in MTB strains, and examined the differences between sensitive MTB and MDR MTB using next generation sequencing (NGS) with Illumina Deep Sequencing technology to better understand the mechanisms of resistance in MDR MTB, A total of 5, 785 and 195, and 6, 290 and 595 qualified Illumina reads were obtained from two MDR MTB strains, and 6, 673 and 665, and 7, 210 and 217 qualified Illumina reads were obtained from two sensitive MTB strains. The overall de novo assembly of miRNA sequence data generated 62 and 62, and 95 and 112 miRNAs between the 18 and 30 bp long from sensitive MTB strains and MDR MTB strains, respectively. Comparative miRNA analysis revealed that 142 miRNAs were differentially expressed in the MDR MTB strain, compared with the sensitive MTB strain, of which 48 were upregulated and 94 were downregulated. There were six similarly expressed miRNAs between the MDR and sensitive MTB strains, and 108 miRNAs were expressed only in the MDR MTB strain. The present study acquired miRNA data from sensitive MTB and MDR MTB strains using NGS techniques, and this identification miRNAs may serve as an invaluable resource for revealing the molecular basis of the regulation of expression associated with the mechanism of drug‑resistance in MTB.
由耐多药结核分枝杆菌（MDR MTB）引起的结核病感染仍是全球重大的公共卫生问题。MDR MTB菌株出现的调控机制仍有待充分阐明，需要进一步研究以制定更好的结核病控制策略。本研究调查了MTB菌株中微小RNA（miRNA）的表达谱，并使用Illumina深度测序技术通过下一代测序（NGS）检查了敏感MTB和MDR MTB之间的差异，以更好地了解MDR MTB的耐药机制。从两株MDR MTB菌株中分别获得了5,785和195条，以及6,290和595条合格的Illumina读数，从两株敏感MTB菌株中分别获得了6,673和665条，以及7,210和217条合格的Illumina读数。miRNA序列数据的整体从头组装分别从敏感MTB菌株和MDR MTB菌株中产生了62和62条，以及95和112条长度在18至30 bp之间的miRNA。比较miRNA分析显示，与敏感MTB菌株相比，MDR MTB菌株中有142条miRNA差异表达，其中48条上调，94条下调。MDR和敏感MTB菌株之间有6条相似表达的miRNA，108条miRNA仅在MDR MTB菌株中表达。本研究使用NGS技术从敏感MTB和MDR MTB菌株中获取了miRNA数据，这些鉴定出的miRNA可能是揭示与MTB耐药机制相关的表达调控分子基础的宝贵资源。
